Inst. of Wood Based Products and Technologies, Univ. of Sopron, Bajcsy-Zsilinszky u. 4, Sopron 9400, Hungary.
Inst. of Applied Arts, Univ. of Sopron, Bajcsy-Zsilinszky u. 4, Sopron 9400, Hungary.
J Food Sci. 2018 Mar;83(3):700-710. doi: 10.1111/1750-3841.14075. Epub 2018 Feb 13.
Ultrasonication was used to extract bioactive compounds from Cannabis sativa L. such as polyphenols, flavonoids, and cannabinoids. The influence of 3 independent factors (time, input power, and methanol concentration) was evaluated on the extraction of total phenols (TPC), flavonoids (TF), ferric reducing ability of plasma (FRAP) and the overall yield. A face-centered central composite design was used for statistical modelling of the response data, followed by regression and analysis of variance in order to determine the significance of the model and factors. Both the solvent composition and the time significantly affected the extraction while the sonication power had no significant impact on the responses. The response predictions obtained at optimum extraction conditions of 15 min time, 130 W power, and 80% methanol were 314.822 mg GAE/g DW of TPC, 28.173 mg QE/g DW of TF, 18.79 mM AAE/g DW of FRAP, and 10.86% of yield. A good correlation was observed between the predicted and experimental values of the responses, which validated the mathematical model. On comparing the ultrasonic process with the control extraction, noticeably higher values were obtained for each of the responses. Additionally, ultrasound considerably improved the extraction of cannabinoids present in Cannabis.
Low frequency ultrasound was employed to extract bioactive compounds from the inflorescence part of Cannabis. The responses evaluated were-total phenols, flavonoids, ferric reducing assay and yield. The solvent composition and time significantly influenced the extraction process. Appreciably higher extraction of cannabinoids was achieved on sonication against control.
超声处理用于从大麻属植物中提取生物活性化合物，如多酚、类黄酮和大麻素。考察了 3 个独立因素（时间、输入功率和甲醇浓度）对总酚（TPC）、类黄酮（TF）、血浆铁还原能力（FRAP）和总收率提取的影响。采用中心复合面设计对面心复合设计进行统计建模，然后进行回归和方差分析，以确定模型和因素的显著性。溶剂组成和时间对提取有显著影响，而超声功率对响应无显著影响。在最佳提取条件（15 分钟时间、130 W 功率和 80%甲醇）下，获得的响应预测值为 314.822 mg GAE/g DW 的 TPC、28.173 mg QE/g DW 的 TF、18.79 mM AAE/g DW 的 FRAP 和 10.86%的收率。响应的预测值与实验值之间观察到良好的相关性，验证了数学模型。与对照提取相比，每个响应的超声处理都得到了明显更高的值。此外，超声还显著提高了大麻中存在的大麻素的提取。
低频超声用于从大麻属植物的花序部分提取生物活性化合物。评估的响应为总酚、类黄酮、铁还原测定和收率。溶剂组成和时间对提取过程有显著影响。与对照相比，超声处理可显著提高大麻素的提取率。
