Department of Orthopaedics, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng 252000, Shandong, China.
Department of Spine Surgery, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China.
Biosci Rep. 2018 Mar 9;38(2). doi: 10.1042/BSR20180064. Print 2018 Apr 27.
Mechanical stimuli obviously affect disc nucleus pulposus (NP) biology. Previous studies have indicated that static compression exhibits detrimental effects on disc biology compared with dynamic compression. To study disc NP cell senescence under static compression and dynamic compression in a disc organ culture, porcine discs were cultured and subjected to compression (static compression: 0.4 MPa for 4 h once per day; dynamic compression: 0.4 MPa at a frequency of 1.0 Hz for 4 h once per day) for 7 days using a self-developed mechanically active bioreactor. The non-compressed discs were used as controls. Compared with the dynamic compression, static compression significantly promoted disc NP cell senescence, reflected by the increased senescence-associated β-galactosidase (SA-β-Gal) activity, senescence-associated heterochromatic foci (SAHF) formation and senescence markers expression, and the decreased telomerase (TE) activity and NP matrix biosynthesis. Static compression accelerates disc NP cell senescence compared with the dynamic compression in a disc organ culture. The present study provides that acceleration of NP cell senescence may be involved in previously reported static compression-mediated disc NP degenerative changes.
机械刺激显然会影响椎间盘核（NP）的生物学特性。先前的研究表明，与动态压缩相比，静态压缩对椎间盘生物学具有有害影响。为了在椎间盘器官培养中研究静态压缩和动态压缩下椎间盘 NP 细胞衰老，使用自行开发的机械活性生物反应器培养猪椎间盘，并对其进行压缩（静态压缩：每天 0.4 MPa 压缩 4 小时；动态压缩：每天 0.4 MPa 频率为 1.0 Hz 压缩 4 小时）7 天。未压缩的椎间盘用作对照。与动态压缩相比，静态压缩通过增加衰老相关β-半乳糖苷酶（SA-β-Gal）活性、衰老相关异染色质焦点（SAHF）形成和衰老标志物表达，以及降低端粒酶（TE）活性和 NP 基质生物合成，显著促进椎间盘 NP 细胞衰老。在椎间盘器官培养中，与动态压缩相比，静态压缩加速了椎间盘 NP 细胞衰老。本研究表明，NP 细胞衰老的加速可能与先前报道的静态压缩介导的椎间盘 NP 退行性变有关。
