Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia.
Department of Dairy Production, University of Khartoum, Khartoum North 13314, Khartoum, Sudan.
Molecules. 2018 Feb 13;23(2):398. doi: 10.3390/molecules23020398.
Tempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian () fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing strains from tempoyak for potential use as probiotics. Seven isolates (DUR2, DUR4, DUR5, DUR8, DUR12, DUR18, and DUR20) out of 44 were able to produce EPS, and exhibited resistance to acid and bile salt compared to the reference strains (ATCC53103) and (ATCC8014). The seven isolated strains belonged to five different species-, , , , and which were identified using API 50 CHL and 16S rRNA gene sequences (Polymerase chain reaction, PCR - based). The seven strains displayed different ability to produce EPS (100-850 mg/L). Isolates exhibited a high survivability to acid (pH 3.0), bile salts (0.3%), and gastrointestinal tract model (<70%). Results showed that the auto-aggregation and cell surface hydrophobicity ranged from 39.98% to 60.09% and 50.80% to 80.53%, respectively, whereas, the highest co-aggregation value (66.44%) was observed by (DUR8) with . The isolates showed good inhibitory activity against tested pathogens, high antioxidant activity (32.29% to 73.36%), and good ability to reduce cholesterol (22.55% to 75.15%). Thus, the seven tested strains have value as probiotics.
榴莲泥是一种功能性马来西亚食品（一种酸发酵调味料），由榴莲果肉制成。本研究旨在从榴莲泥中分离和鉴定潜在的产胞外多糖（EPS）菌株，以作为潜在的益生菌使用。在 44 株中，有 7 株（DUR2、DUR4、DUR5、DUR8、DUR12、DUR18 和 DUR20）能够产生 EPS，并且与参考菌株（ATCC53103）和（ATCC8014）相比，表现出对酸和胆汁盐的抗性。这 7 株分离株属于 5 个不同的种，即、、、和，使用 API 50 CHL 和 16S rRNA 基因序列（聚合酶链反应（PCR））进行鉴定。这 7 株菌表现出不同的产 EPS 能力（100-850mg/L）。分离株在胃酸（pH3.0）、胆汁盐（0.3%）和胃肠道模型（<70%）中具有高存活率。结果表明，自动聚集和细胞表面疏水性分别为 39.98%-60.09%和 50.80%-80.53%，而（DUR8）与的共聚集值最高（66.44%）。分离株对测试病原体表现出良好的抑制活性、高抗氧化活性（32.29%-73.36%）和良好的降胆固醇能力（22.55%-75.15%）。因此，这 7 株测试菌株具有作为益生菌的价值。
