Department of Food Science and Technology, Faculty of Agricultural Technology, IPB University (Bogor Agricultural University), Bogor, West Java, Indonesia.
Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center, IPB University (Bogor Agricultural University), Bogor, West Java, Indonesia.
J Microbiol Biotechnol. 2019 May 28;30(5):726-732. doi: 10.4014/jmb.1910.10028.
Indonesian kefir grains are potential sources of lactic acid bacteria (LAB) that may act as probiotics with specific functional properties. In this study we explored the potential of the probiotic and cholesterol-lowering effect of LAB isolated from Indonesian kefir grains obtained from Bogor, Bandung, Jakarta, and Yogyakarta. The results revealed that 10 isolates showed considerable survivability at low pH and bile salt with total cell reduction of ~3 log colony-forming units per milliliter after exposure to pH 2.5 and 0.5% (w/v) bile salt for 1 and 3 h, respectively. All strains exhibited strong antimicrobial activities against pathogenic bacteria and were sensitive to a wide spectrum of antibiotics but exhibited weak bile salt hydrolase activity. Identification based on 16S RNA suggested that nine isolates were and one was . The ability of the isolates to reduce cholesterol from the media varied, ranging from 22.08% to 68.75% with the highest reduction shown by JK17. The ability to remove cholesterol from the media decreased greatly in resting and dead cells, ranging from 14.58% to 22.08% in resting cells and from 7.89% to 18.17% in dead cells. It can be concluded that Indonesian kefir grains contain LAB potentially acting as probiotics capable of reducing cholesterol. The cholesterol-lowering effect especially occurs when the cells are metabolically active.
印度尼西亚开菲尔粒是乳酸菌（LAB）的潜在来源，这些乳酸菌可能具有特定的功能特性，作为益生菌发挥作用。在这项研究中，我们探索了从茂物、万隆、雅加达和日惹获得的印度尼西亚开菲尔粒中分离出的 LAB 的益生菌潜力和降胆固醇作用。结果表明，10 株分离株在低 pH 值和胆汁盐条件下具有相当的存活率，在 pH 2.5 和 0.5%（w/v）胆汁盐暴露 1 和 3 h 后，总细胞减少约 3 个对数菌落形成单位/毫升。所有菌株对致病菌均表现出强烈的抗菌活性，对广谱抗生素敏感，但胆汁盐水解酶活性较弱。基于 16S RNA 的鉴定表明，9 株分离株为 ，1 株为 。分离株从培养基中降低胆固醇的能力各不相同，范围从 22.08%到 68.75%，其中 JK17 的降低幅度最高。在静止和死亡细胞中，从培养基中去除胆固醇的能力大大降低，在静止细胞中为 14.58%至 22.08%，在死亡细胞中为 7.89%至 18.17%。可以得出结论，印度尼西亚开菲尔粒含有潜在的作为益生菌的乳酸菌，能够降低胆固醇。降胆固醇作用尤其发生在细胞代谢活跃时。
