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筛选具有潜在益生菌活性的亚群以抑制断奶仔猪 ETEC K88。

Screening of Subsp. with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets.

机构信息

Henan Key Lab Ion Beam Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, China.

School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450052, China.

出版信息

Molecules. 2020 Sep 29;25(19):4481. doi: 10.3390/molecules25194481.

DOI:10.3390/molecules25194481
PMID:33003556
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7582832/
Abstract

For screening excellent lactic acid bacteria (LAB) strains to inhibit enterotoxigenic (ETEC) K88, inhibitory activities of more than 1100 LAB strains isolated from different materials, and kept in the lab, were evaluated in this study. Nine strains with inhibition zones, at least 22.00 mm (including that of a hole puncher, 10.00 mm), and good physiological and biochemical characteristics identified by 16S DNA gene sequencing and A gene multiple detection, were assigned to () subsp. (5), (1), (1), (1) and (1), respectively. As investigated for their tolerance abilities and safety, only strain ZA3 possessed high hydrophobicity and auto-aggregation abilities, had high survival rate in low pH, bile salt environment, and gastrointestinal (GI) fluids, was sensitive to ampicillin, and resistant to norfloxacin and amikacin, without hemolytic activity, and did not carry antibiotic resistance genes, but exhibited broad spectrum activity against a wide range of microorganisms. Antibacterial substance may attribute to organic acids, especially lactic acid and acetic acid. The results indicated that the selected strain subsp. ZA3 could be considered a potential probiotic to inhibit ETEC K88 in weaned piglets for further research.

摘要

为了筛选出能够抑制肠致病性大肠杆菌（ETEC）K88 的优良乳酸菌（LAB）菌株，本研究评估了从不同材料中分离并保存在实验室中的 1100 多个 LAB 菌株的抑制活性。有 9 株具有至少 22.00mm 的抑菌圈（包括打孔器的 10.00mm），并且通过 16S DNA 基因测序和 A 基因多重检测鉴定出良好的生理生化特性，分别被分配到 () 亚种。（5）、（1）、（1）、（1）和 （1）。对其耐受性和安全性进行研究，只有菌株 ZA3 具有较高的疏水性和自动聚集能力，在低 pH 值、胆盐环境和胃肠道（GI）液中有较高的存活率，对氨苄西林敏感，对诺氟沙星和阿米卡星耐药，无溶血活性，且不携带抗生素耐药基因，但对多种微生物具有广谱活性。抗菌物质可能归因于有机酸，特别是乳酸和乙酸。结果表明，所选的 亚种 ZA3 菌株可以被认为是一种潜在的益生菌，用于进一步研究抑制断奶仔猪 ETEC K88。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/a12038624918/molecules-25-04481-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/efd5667f0459/molecules-25-04481-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/65202e286116/molecules-25-04481-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/418a50125f07/molecules-25-04481-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/bd43afdca915/molecules-25-04481-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/898910c65d12/molecules-25-04481-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/a44c1a43a792/molecules-25-04481-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/d528d311c089/molecules-25-04481-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/3f3318d11803/molecules-25-04481-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/4830e2a01bd1/molecules-25-04481-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/a12038624918/molecules-25-04481-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/efd5667f0459/molecules-25-04481-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/65202e286116/molecules-25-04481-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/418a50125f07/molecules-25-04481-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/bd43afdca915/molecules-25-04481-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/898910c65d12/molecules-25-04481-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/a44c1a43a792/molecules-25-04481-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/d528d311c089/molecules-25-04481-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/3f3318d11803/molecules-25-04481-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/4830e2a01bd1/molecules-25-04481-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d71a/7582832/a12038624918/molecules-25-04481-g010.jpg

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