College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
Mar Pollut Bull. 2020 Feb;151:110812. doi: 10.1016/j.marpolbul.2019.110812. Epub 2019 Dec 10.
A fluorescence probe was delicately designed for the detection of malachite green (MG) in water and fish samples. Through the electrostatic self-assembly of CdTe QDs on the surface of polystyrene (PS) microspheres, the fluorescence signal was amplified. After grafting molecularly imprinted film, the fluorescence probe of MIP@PS@CdTe was fabricated and applied to the detection of MG based on fluorescence quenching. The linear range of MG detection was 0.01-20 μmol L, and the detection limit was 4.7 nmol L (3σ, n = 9) which was much lower than those of the previous reports. The recoveries of MG in aquaculture water and fish samples ranging from 87.6% to 105.4% illustrated that the detection by MIP@PS@CdTe probe was accurate and reliable.
设计了一种荧光探针用于检测水样和鱼样中的孔雀石绿（MG）。通过 CdTe QDs 在聚苯乙烯（PS）微球表面的静电自组装，放大了荧光信号。接枝分子印迹膜后，制备了 MIP@PS@CdTe 荧光探针，并基于荧光猝灭法用于 MG 的检测。MG 的检测线性范围为 0.01-20 μmol·L，检测限为 4.7 nmol·L（3σ，n=9），远低于以往的报道。在水产养殖用水和鱼样中的 MG 回收率在 87.6%到 105.4%之间，表明 MIP@PS@CdTe 探针的检测准确可靠。
