Farrell P A, Mikines K J, Bach F W, Sonne B, Galbo H
Life Sci. 1986 Sep 15;39(11):965-71. doi: 10.1016/0024-3205(86)90284-5.
Seven healthy untrained men were studied to determine if sustained hyperglycemia is a stimulus to enhanced plasma levels of beta endorphin (beta-EP) and if so whether prior exercise affects that enhancement. After an overnight fast hyperglycemic glucose clamps were performed on 3 separate days: after prior rest, 2 h after exercise, and 48 h after exercise. Subjects exercised on a bicycle ergometer for 1 h at 150 W (64% VO2 max). Plasma glucose concentration was elevated in 4 continuous sequential stages to 7, 11, 20 and 35 mM with each stage lasting 90 min. Plasma glucose concentrations did not differ for each subject across the three clamps. beta-EP immunoreactivity was measured in arterialized venous blood samples using a specific and sensitive radioimmunoassay. Resting beta-EP at basal glucose concentrations was 3.8 +/- 0.7 fmol X ml-1 (mean +/- se) and prior exercise either 2h (3.2 +/- 0.5 fmol X ml-1) or 48 h (4.3 +/- 0.7 fmol X ml-1) before a clamp study did not effect these levels, (p greater than 0.05). At no time during the 3 hyperglycemic clamps did plasma levels of beta-EP differ significantly from resting values. At the highest level of hyperglycemia (35 mM) beta-EP was 3.1 +/- 0.2, 4.9 +/- 0.6 and 4.8 +/- 0.7 fmol X ml-1 in the resting, 2h and 48 h post exercise clamp studies respectively. The significance of these data is that this lack of a response is in distinct contrast to elevations of this peptide found during hypoglycemic states. We conclude that sustained hyperglycemia is not a stimulus to enhanced secretion of beta-EP into plasma and this lack of a response is not effected by prior exercise.
对7名未经训练的健康男性进行了研究，以确定持续性高血糖是否会刺激β-内啡肽（β-EP）血浆水平升高，如果是，先前的运动是否会影响这种升高。在禁食过夜后，在3个不同的日子进行了高血糖葡萄糖钳夹试验：在先前休息后、运动后2小时以及运动后48小时。受试者在自行车测力计上以150W（64%最大摄氧量）运动1小时。血浆葡萄糖浓度在4个连续阶段升高至7、11、20和35mM，每个阶段持续90分钟。每个受试者在三次钳夹试验中的血浆葡萄糖浓度没有差异。使用特异性和灵敏的放射免疫分析法在动脉化静脉血样本中测量β-EP免疫反应性。基础葡萄糖浓度下的静息β-EP为3.8±0.7fmol×ml-1（平均值±标准误），在钳夹研究前2小时（3.2±0.5fmol×ml-1）或48小时（4.3±0.7fmol×ml-1）的先前运动并未影响这些水平，（p>0.05）。在3次高血糖钳夹试验期间，β-EP的血浆水平在任何时候都与静息值无显著差异。在高血糖最高水平（35mM）时，静息、运动后2小时和运动后48小时钳夹试验中的β-EP分别为3.1±0.2、4.9±0.6和4.8±0.7fmol×ml-1。这些数据的意义在于，这种无反应与低血糖状态下发现的该肽升高形成明显对比。我们得出结论，持续性高血糖不是刺激β-EP分泌到血浆中增加的因素，并且这种无反应不受先前运动的影响。
