Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, Beijing, China.
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China.
Infect Immun. 2018 Apr 23;86(5). doi: 10.1128/IAI.00073-18. Print 2018 May.
Coccidiosis, caused by different species of parasites, is an economically important disease of poultry and livestock worldwide. Here we report previously unknown alterations in the gut microbes and metabolism of BALB/c mice infected with Specifically, we observed a significant shift in the abundance of cecal bacteria and disrupted metabolism in parasitized animals. The relative abundances of bacterium NK4A136, , , and declined in response to infection, whereas , , , , and were increased. Carbohydrate and amino acid metabolites in the serum samples of infected mice were significantly altered compared to naïve controls. Levels of amino acids, including asparagine, histidine, l-cysteine, tryptophan, lysine, glycine, serine, alanine, proline, ornithine, methionine, and valine, decreased on day 7 postinfection before returning to baseline on day 14. In addition, increased levels of indolelactate and mannitol and a reduced amount of oxalic acid indicated impaired carbon metabolism upon parasitic infection. These data demonstrate that intestinal coccidial infection perturbs the microbiota and disrupts carbon and nitrogen metabolism.
球虫病是由不同种寄生虫引起的，是一种在全世界范围内对家禽和家畜具有重要经济意义的疾病。在这里，我们报告了以前未知的感染 对 BALB/c 小鼠肠道微生物群和代谢的改变。具体来说，我们观察到感染寄生虫的动物盲肠细菌丰度和代谢发生了显著变化。 、 、 、 和 的相对丰度因 感染而下降，而 、 、 、 、 和 则增加。与未感染的对照相比，感染小鼠的血清样本中的碳水化合物和氨基酸代谢物发生了显著改变。感染后第 7 天，包括天冬酰胺、组氨酸、l-半胱氨酸、色氨酸、赖氨酸、甘氨酸、丝氨酸、丙氨酸、脯氨酸、精氨酸、甲硫氨酸和缬氨酸在内的氨基酸水平下降，然后在第 14 天恢复到基线。此外，吲哚乳酸和甘露醇水平升高，草酸水平降低，表明寄生虫感染后碳代谢受损。这些数据表明，肠道球虫感染扰乱了微生物群，并破坏了碳和氮代谢。
