非模式原生动物的基因操作：进展、挑战与未来展望。
Genetic manipulation for the non-model protozoan : Advancements, challenges, and future perspective.
作者信息
Li Yaru, Suo Jingxia, Liang Ruiying, Liang Lin, Liu Xianyong, Ding Jiabo, Suo Xun, Tang Xinming
机构信息
Key Laboratory of Animal Biosafety Risk Prevention and Control (North) & Key Laboratory of Veterinary Biological Products and Chemical Drugs of MARA, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
National Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology of the MARA, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
出版信息
iScience. 2025 Feb 17;28(3):112060. doi: 10.1016/j.isci.2025.112060. eCollection 2025 Mar 21.
parasites pose a significant global threat to animal health, necessitating improved and cost-effective control measures. Genetic manipulation is pivotal for understanding biology and designing targeted control strategies. Recent advancements, including genome sequencing and the development of transient and stable transfection systems, have significantly enhanced insights into the molecular biology of . These advancements have paved the way for cutting-edge techniques like CRISPR-Cas9 gene editing. This review summarizes the key milestones in the development of genetic manipulation platforms for and their transformative applications, such as the development of next-generation drugs, vaccines, and -based vaccine vectors. Furthermore, this review provides insights that could be applicable to the establishment of genetic tools for other protozoan organisms.
寄生虫对动物健康构成了重大的全球威胁，因此需要改进且具有成本效益的控制措施。基因操作对于理解生物学和设计针对性的控制策略至关重要。包括基因组测序以及瞬时和稳定转染系统的开发在内的最新进展，显著增强了对[未明确的生物]分子生物学的认识。这些进展为CRISPR-Cas9基因编辑等前沿技术铺平了道路。本综述总结了[未明确的生物]基因操作平台开发中的关键里程碑及其变革性应用，如新一代药物、疫苗以及基于[未明确的生物]的疫苗载体的开发。此外，本综述还提供了可应用于为其他原生动物建立遗传工具的见解。
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