The Affiliated Eye Hospital, Wenzhou Medical University, Wenzhou, China.
Department of Ophthalmology and Center for Creative Biomedical Scientists, Chonnam National University, Gwangju, Republic of Korea.
Oxid Med Cell Longev. 2017;2017:4523673. doi: 10.1155/2017/4523673. Epub 2017 Dec 26.
To investigate the effects of (CO) on human corneal epithelial (HCE) cells, a murine experimental dry eye (EDE) model, and the efficacy of antioxidant eye mask in dry eye disease (DED) patients.
0.001%, 0.01%, and 0.1% CO extracts were used to treat HCE cells, cell viability, and production of antioxidative enzymes, and reactive oxygen species (ROS) were assessed. Afterwards, CO extracts or balanced salt solution (BSS) was applied in EDE. Clinical and experimental parameters were measured at 7 days after treatment. In addition, DED patients were randomly assigned to wear either an eye mask containing CO extracts or a placebo. Clinical parameters were evaluated.
The viability of HCE cells and antioxidative enzyme expression significantly improved after treatment with 0.1% CO extracts. Mice treated with 0.1% CO extracts showed significant improvement in clinical parameters. During the trial, the clinical parameters significantly improved in the treatment group at 4 weeks after application.
0.1% CO extracts could promote the expression of antioxidative proteins and ROS production. In addition, an eye mask containing CO extracts could improve DED clinical parameters. These suggest that CO extracts may be useful as an adjunctive option for the DED treatment.
研究（CO）对人眼角膜上皮（HCE）细胞的影响，建立实验性干眼（EDE）小鼠模型，评估抗氧化眼罩治疗干眼疾病（DED）患者的疗效。
用 0.001%、0.01%和 0.1%CO 提取物处理 HCE 细胞，评估细胞活力和抗氧化酶的产生以及活性氧（ROS）。然后，在 EDE 中应用 CO 提取物或平衡盐溶液（BSS）。治疗 7 天后测量临床和实验参数。此外，DED 患者随机分为佩戴含有 CO 提取物的眼罩或安慰剂。评估临床参数。
用 0.1%CO 提取物处理后，HCE 细胞活力和抗氧化酶表达显著提高。用 0.1%CO 提取物治疗的小鼠临床参数显著改善。在试验过程中，治疗组在应用后 4 周时临床参数显著改善。
0.1%CO 提取物可促进抗氧化蛋白的表达和 ROS 的产生。此外，含有 CO 提取物的眼罩可改善 DED 的临床参数。这些表明 CO 提取物可能是 DED 治疗的一种有用的辅助选择。
