Nakamura R M, Nakamura Y, Nagayama A, Tokunaga T
Surv Synth Pathol Res. 1985;4(5-6):443-56. doi: 10.1159/000156995.
Antigen-specific Ts against DTH to BCG were induced in vitro by culturing the splenic T cells of C3H/He mice together with the lined macrophage, SL-1, pulsed with PPD. The suppressor activity was determined by the suppression of DTH in CY-treated syngeneic recipients which had received the cultured cells before BCG sensitization. SL-1 cells express I-A and I-J antigens on the surface, while SL-4 cells, which originated in the same mouse strain as SL-1, do not express any Ia antigens. When I-A antigens on SL-1 were blocked by anti-I-A antibody without complement before PPD-pulsing, the induction of Ts was rather enhanced. When I-J antigens on SL-1 were blocked by anti-I-J alloantiserum, no Ts were induced. Instead, these I-J-blocked and PPD-pulsed SL-1 cells induced effector cells of DTH in vitro. Ia-negative lined macrophage SL-4 cells did not show any activity either in suppressor or in effector cell induction. From these results, we propose a model for the immune modulation led by APC.
通过将C3H/He小鼠的脾T细胞与用PPD脉冲处理的贴壁巨噬细胞SL-1一起培养，在体外诱导出针对卡介苗迟发型超敏反应（DTH）的抗原特异性Ts细胞。通过在卡介苗致敏前接受培养细胞的经环磷酰胺（CY）处理的同基因受体中DTH的抑制来确定抑制活性。SL-1细胞在表面表达I-A和I-J抗原，而起源于与SL-1相同小鼠品系的SL-4细胞不表达任何Ia抗原。当在PPD脉冲处理前用抗I-A抗体而非补体阻断SL-1上的I-A抗原时，Ts细胞的诱导反而增强。当用抗I-J同种异型抗血清阻断SL-1上的I-J抗原时，未诱导出Ts细胞。相反，这些I-J阻断且经PPD脉冲处理的SL-1细胞在体外诱导出DTH效应细胞。Ia阴性的贴壁巨噬细胞SL-4细胞在抑制细胞或效应细胞诱导方面均未表现出任何活性。根据这些结果，我们提出了一个由抗原呈递细胞（APC）介导的免疫调节模型。
