Chen Wen-Ming, Chen Yi-Ling, Li Yi-Shu, Sheu Shih-Yi
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung, 811, Taiwan.
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung, 811, Taiwan.
Arch Microbiol. 2018 Jul;200(5):811-817. doi: 10.1007/s00203-018-1492-4. Epub 2018 Feb 13.
Strain TTM-94, isolated from a water sample taken from the Caohu River in Taiwan, was characterized using a polyphasic taxonomic approach. Cells of strain TTM-94 were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate-accumulating, motile by a single polar flagellum, short rod-shaped and surrounded by a thick capsule and it formed cream colored colonies. Growth occurred at 20-30 °C (optimum, 30 °C), at pH 6.0-8.0 (optimum, pH 6.0), and in the presence of 0-2% NaCl (optimum 0.5%). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TTM-94 belonged to the genus Aquincola in the Rubrivivax-Roseateles-Leptothrix-Ideonella-Aquabacterium branch of the class Betaproteobacteria and its most closely related neighbour was Aquincola tertiaricarbonis L10 with sequence similarity of 97.0%. Strain TTM-94 contained summed feature 3 (comprising Cω7c and/or Cω6c), C and Cω7c as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized lipids. The major respiratory quinone was Q-8. Genomic DNA G + C content of strain TTM-94 was 70.7 mol%. Strain TTM-94 exhibited less than 30% DNA-DNA relatedness with A. tertiaricarbonis L10. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain TTM-94 should be classified as a novel species of the genus Aquincola, for which the name Aquincola amnicola sp. nov. is presented. The type strain is TTM-94 (= BCRC 80890 = LMG 28709).
从台湾草湖河采集的水样中分离出的TTM-94菌株，采用多相分类方法进行了特征描述。TTM-94菌株的细胞革兰氏染色阴性，好氧，能积累聚-β-羟基丁酸，通过单根极生鞭毛运动，呈短杆状，被厚荚膜包围，形成奶油色菌落。在20-30°C（最适温度为30°C）、pH 6.0-8.0（最适pH为6.0）以及0-2% NaCl（最适浓度为0.5%）存在的条件下可生长。基于16S rRNA基因序列的系统发育分析表明，TTM-94菌株属于β-变形菌纲红环菌-玫瑰色菌-纤发菌-艾氏菌-水生杆菌分支中的Aquincola属，与其亲缘关系最密切的近缘种是Aquincola tertiaricarbonis L10，序列相似性为97.0%。TTM-94菌株以特征性脂肪酸总和3（包含Cω7c和/或Cω6c）、C和Cω7c作为主要脂肪酸。极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油和几种未鉴定的脂质组成。主要呼吸醌为Q-8。TTM-94菌株的基因组DNA G + C含量为70.7 mol%。TTM-94菌株与A. tertiaricarbonis L10的DNA-DNA相关性低于30%。不同的表型特征以及系统发育推断表明，TTM-94菌株应被归类为Aquincola属的一个新物种，为此提出名为Aquincola amnicola sp. nov.。模式菌株为TTM-94（= BCRC 80890 = LMG 28709）。
