Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan, ROC.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1810-1816. doi: 10.1099/ijs.0.043729-0. Epub 2012 Sep 14.
A slightly thermophilic bacterial strain, designated AT-A2(T), was isolated from a hot spring water sample taken from the Antun hot spring in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain AT-A2(T) were aerobic, Gram-negative, motile by a single polar flagellum and formed non-pigmented colonies. Growth occurred at 35-60 °C (optimum, 55 °C), with 0-1.0 % NaCl (optimum, 0.2 %) and at pH 7.0-9.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AT-A2(T) belonged to the genus Tepidimonas and its closest neighbour was Tepidimonas thermarum AA-1(T) with a sequence similarity of 97.5 %. The predominant cellular fatty acids were C16 : 0 (40.2 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 20.1 %) and C17 : 0 cyclo (11.5 %). The major respiratory quinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and several uncharacterized phospholipids. The DNA G+C content of strain AT-A2(T) was 70.1 mol%. The mean level of DNA-DNA relatedness between strain AT-A2(T) and Tepidimonas thermarum AA-1(T) was 23.9 %. On the basis of the phylogenetic and phenotypic data, strain AT-A2(T) should be classified as representing a novel species, for which the name Tepidimonas fonticaldi sp. nov. is proposed. The type strain is AT-A2(T) ( = LMG 26746(T) = KCTC 23862(T) = BCRC 80391(T)).
从台湾安通温泉采集的温泉水样中分离到一株嗜热细菌菌株，命名为 AT-A2(T)，并采用多相分类法对其进行了表征。菌株 AT-A2(T)的细胞为需氧、革兰氏阴性、单极鞭毛运动，形成非色素菌落。该菌株在 35-60°C（最佳温度为 55°C）、0-1.0%NaCl（最佳浓度为 0.2%）和 pH7.0-9.0（最佳 pH 值为 7.0）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 AT-A2(T)属于 Tepidimonas 属，与最接近的亲缘种 Tepidimonas thermarum AA-1(T)的序列相似性为 97.5%。主要的细胞脂肪酸为 C16:0（40.2%）、特征性脂肪酸 3（C16:1ω7c 和/或 C16:1ω6c；20.1%）和 C17:0 环（11.5%）。主要的呼吸醌为 Q-8。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、一种未鉴定的胺脂和几种未鉴定的磷脂组成。菌株 AT-A2(T)的 DNA G+C 含量为 70.1mol%。菌株 AT-A2(T)与 Tepidimonas thermarum AA-1(T)之间的 DNA-DNA 同源性平均值为 23.9%。基于系统发育和表型数据，菌株 AT-A2(T)应被分类为代表一个新种，因此建议将其命名为 Tepidimonas fonticaldi sp. nov.。其模式菌株为 AT-A2(T)（=LMG 26746(T)=KCTC 23862(T)=BCRC 80391(T)）。
