Ozkan Ozan, Sasmazel Hilal Turkoglu
Bioengineering Division, Hacettepe University, Beytepe, Ankara, 06800, Turkey.
Department of Metallurgical and Materials Engineering, Atilim University, Incek, Golbasi, Ankara, 06836, Turkey.
J Nanosci Nanotechnol. 2018 Apr 1;18(4):2415-2421. doi: 10.1166/jnn.2018.14378.
In this study, antibacterial performance of the coaxially electrospun Poly-ε-caprolactone (PCL)-chitosan core-shell scaffolds developed, optimized and identified physically and chemically in our previous study, were evaluated for the suitability in wound healing applications. The aim of utilizing a core-shell fibrous scaffold with PCL as core and chitosan as shell was to combine natural biocompatibility, biodegradability and antibacterial properties of chitosan with mechanical properties and resistance to enzymatic degradation of PCL. The scaffolds were prepared with the optimized parameters, obtained from our previous study. Thickness and contact angle measurements as well as Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) analyses confirmed repeated fabrication of PCL-chitosan core-shell scaffolds. In this study, assays specific to wound dressing materials, such as water vapor transmission rate (WVTR), in vitro degradability and antibacterial tests were carried out. WVTR value of PCL-chitosan core-shell scaffolds was higher (2315 ± 3.4 g/m2 · day) compared to single PCL scaffolds (1654 ± 3.2 g/m2 · day) due to the higher inter-fiber pore size. Additionally, in vitro degradability assays showed that the susceptibility of chitosan to enzymatic degradation can be significantly improved by hybridization with more resistant PCL while still keeping the scaffold to be considered as biodegradable. Finally, inhibition ratio and inhibition zone measurements showed that the PCL-chitosan core-shell polymeric scaffolds had significant antibacterial performance (52.860 ± 2.298% and 49.333 ± 0.719% inhibition ratios; 13.975 ± 0.124 mm and 12.117 ± 0.133 mm clear inhibition zones, against E. coli and S. aureus, respectively), close to the native chitosan. Therefore, the developed scaffolds can be considered as suitable candidates for biodegradable wound dressing applications.
在本研究中，对我们之前研究中开发、优化并进行物理和化学鉴定的同轴电纺聚己内酯（PCL）-壳聚糖核壳支架的抗菌性能进行了评估，以确定其在伤口愈合应用中的适用性。使用以PCL为核、壳聚糖为壳的核壳纤维支架的目的是将壳聚糖的天然生物相容性、生物降解性和抗菌性能与PCL的机械性能和抗酶降解性相结合。这些支架是根据我们之前研究中获得的优化参数制备的。厚度和接触角测量以及扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析证实了PCL-壳聚糖核壳支架的重复制备。在本研究中，进行了针对伤口敷料材料的特定试验，如水蒸气透过率（WVTR）、体外降解性和抗菌测试。由于纤维间孔径较大，PCL-壳聚糖核壳支架的WVTR值（2315±3.4 g/m²·天）高于单一PCL支架（1654±3.2 g/m²·天）。此外，体外降解性试验表明，壳聚糖与更具抗性的PCL杂交可显著提高其对酶降解的敏感性，同时仍使支架被视为可生物降解。最后，抑制率和抑菌圈测量表明，PCL-壳聚糖核壳聚合物支架具有显著的抗菌性能（对大肠杆菌和金黄色葡萄球菌的抑制率分别为52.860±2.298%和49.333±0.719%；清晰抑菌圈分别为13.975±0.124 mm和12.117±0.133 mm），接近天然壳聚糖。因此，所开发的支架可被视为可生物降解伤口敷料应用的合适候选材料。
