Egorikhina Marfa N, Bronnikova Irina I, Rubtsova Yulia P, Charykova Irina N, Bugrova Marina L, Linkova Daria D, Aleynik Diana Ya
Federal State Budgetary Educational Institution of Higher Education, Privolzhsky Research Medical University of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia.
Polymers (Basel). 2021 Oct 10;13(20):3470. doi: 10.3390/polym13203470.
The success of the regenerative process resulting from the implantation of a scaffold or a tissue-engineered structure into damaged tissues depends on a series of factors, including, crucially, the biodegradability of the implanted materials. The selection of a scaffold with appropriate biodegradation characteristics allows for synchronization of the degradation of the construct with the processes involved in new tissue formation. Thus, it is extremely important to characterize the biodegradation properties of potential scaffold materials at the stage of in vitro studies. We have analyzed the biodegradation of hybrid fibrin-collagen scaffolds in both PBS solution and in trypsin solution and this has enabled us to describe the processes of both their passive and enzymatic degradation. It was found that the specific origin of the collagen used to form part of the hybrid scaffolds could have a significant effect on the nature of the biodegradation process. It was also established, during comparative studies of acellular scaffolds and scaffolds containing stem cells, that the cells, too, make a significant contribution to changes in the biodegradation and structural properties of such scaffolds. The study results also provided evidence indicating the dependency between the pre-cultivation period for the cellular scaffolds and the speed and extent of their subsequent biodegradation. Our discussion of results includes an attempt to explain the mechanisms of the changes found. We hope that the said results will make a significant contribution to the understanding of the processes affecting the differences in the biodegradation properties of hybrid, biopolymer, and hydrogel scaffolds.
将支架或组织工程结构植入受损组织后再生过程的成功取决于一系列因素，其中至关重要的是植入材料的生物降解性。选择具有适当生物降解特性的支架可使构建体的降解与新组织形成过程同步。因此，在体外研究阶段表征潜在支架材料的生物降解特性极为重要。我们分析了混合纤维蛋白-胶原蛋白支架在PBS溶液和胰蛋白酶溶液中的生物降解情况，这使我们能够描述其被动降解和酶促降解过程。研究发现，用于构成混合支架一部分的胶原蛋白的特定来源可能对生物降解过程的性质产生重大影响。在对无细胞支架和含干细胞支架的比较研究中还发现，细胞对这类支架的生物降解和结构特性变化也有重大贡献。研究结果还提供了证据，表明细胞支架的预培养期与其随后生物降解的速度和程度之间存在相关性。我们对结果的讨论包括试图解释所发现变化的机制。我们希望上述结果将对理解影响混合、生物聚合物和水凝胶支架生物降解特性差异的过程做出重大贡献。
