Ghasemvand Fariba, Kabiri Mahboubeh, Hassan-Zadeh Vahideh, Simchi Abdolreza
Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran.
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Front Mol Biosci. 2023 Jul 13;10:1220357. doi: 10.3389/fmolb.2023.1220357. eCollection 2023.
Due to the potential positive effects of rosuvastatin (RSV) on human mesenchymal stem cells (MSCs) osteogenesis and new bone regeneration, it is crucial to develop a suitable carrier that can effectively control the release profile of RSV. The primary objective of this study was to introduce a novel drug delivery system based on core/shell nanofibrous structures, enabling a sustained release of RSV. To achieve this, coaxial electrospinning was employed to fabricate chitosan (CS)+polyethylene oxide (PEO)/polycaprolactone (PCL) nanofibrous mats, wherein RSV was incorporated within the core of nanofibers. By optimizing the relevant parameters of the electrospinning process, the mats' surface was further modified using plasma treatment. The fibers' shape, structure, and thermal stability were characterized. The wettability, and degradation properties of the fabricated mats were also examined. studies were conducted to examine the release behavior of RSV. Additionally, the capability of MSCs to survive and differentiate into osteocytes when cultured on nanofibers containing RSV was evaluated. Results demonstrated the successful fabrication of CS + PEO + RSV/PCL core/shell mats with a core diameter of approximately 370 nm and a shell thickness of around 70 nm under optimized conditions. Plasma treatment was found to enhance the wettability and drug-release behavior of the mats. The nanofibrous structure, serving as a carrier for RSV, exhibited increased proliferation of MSCs and enhanced osteogenic differentiation. Therefore, it can be concluded that CS + PEO + RSV/PCL core/shell nanofibrous structure can be utilized as a sustained-release platform for RSV over an extended period, making it a promising candidate for guided bone regeneration.
由于瑞舒伐他汀（RSV）对人间充质干细胞（MSCs）成骨和新骨再生具有潜在的积极作用，因此开发一种能够有效控制RSV释放曲线的合适载体至关重要。本研究的主要目的是引入一种基于核/壳纳米纤维结构的新型药物递送系统，实现RSV的持续释放。为此，采用同轴静电纺丝法制备壳聚糖（CS）+聚环氧乙烷（PEO）/聚己内酯（PCL）纳米纤维垫，其中RSV被包裹在纳米纤维的核内。通过优化静电纺丝工艺的相关参数，利用等离子体处理对垫的表面进行进一步改性。对纤维的形状、结构和热稳定性进行了表征。还研究了制备的垫的润湿性和降解性能。进行了研究以考察RSV的释放行为。此外，评估了MSCs在含有RSV的纳米纤维上培养时存活并分化为骨细胞的能力。结果表明，在优化条件下成功制备了核直径约为370 nm、壳厚度约为70 nm的CS + PEO + RSV/PCL核/壳垫。发现等离子体处理可增强垫的润湿性和药物释放行为。作为RSV载体的纳米纤维结构表现出MSCs增殖增加和成骨分化增强。因此，可以得出结论，CS + PEO + RSV/PCL核/壳纳米纤维结构可作为RSV的长效释放平台，使其成为引导骨再生的有前途的候选材料。
