Millar Daniel, Murphy Larry, Labrie Audrey, Maurer-Spurej Elisabeth
Research & Development, LightIntegra Technology Inc.
Quality Engineering & Regulatory, LightIntegra Technology Inc.
J Vis Exp. 2018 Jan 31(131):56893. doi: 10.3791/56893.
Platelet inventory management based on screening microparticle content in platelet concentrates is a new quality improvement initiative for hospital blood banks. Cells fragment off microparticles (MP) when they are stressed. Blood and blood components may contain cellular fragments from a variety of cells, most notably from activated platelets. When performing their roles as innate immune cells and major players in coagulation and hemostasis, platelets change shape and generate microparticles. With dynamic light scattering (DLS)-based microparticle detection, it is possible to differentiate activated (high microparticle) from non-activated (low microparticle) platelets in transfusions, and optimize the use of this scarce blood product. Previous research suggests that providing non-activated platelets for prophylactic use in hematology-oncology patients could reduce their risk of becoming refractory and improve patient care. The goal of this screening method is to routinely differentiate activated from non-activated platelets. The method described here outlines the steps to be performed for routine platelet inventory management in a hospital blood bank: obtaining a sample from a platelet transfusion, loading the sample into the capillary for DLS measurement, performing the DLS test to identify microparticles, and using the reported microparticle content to identify activated platelets.
基于筛查血小板浓缩物中微粒含量的血小板库存管理是医院血库一项新的质量改进举措。细胞在受到应激时会破碎产生微粒（MP）。血液及血液成分可能含有来自多种细胞的细胞碎片，最显著的是来自活化血小板的碎片。血小板在作为固有免疫细胞以及凝血和止血的主要参与者发挥作用时，会改变形状并产生微粒。通过基于动态光散射（DLS）的微粒检测，可以在输血中区分活化（高微粒）和未活化（低微粒）血小板，并优化这种稀缺血液制品的使用。先前的研究表明，为血液肿瘤患者预防性使用未活化血小板可降低其产生难治性的风险并改善患者护理。这种筛查方法的目标是常规区分活化和未活化血小板。此处描述的方法概述了医院血库中进行常规血小板库存管理应执行的步骤：从血小板输注中获取样本，将样本加载到毛细管中进行DLS测量，进行DLS测试以识别微粒，并使用报告的微粒含量识别活化血小板。
