Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering , Peking University , Beijing 100871 , People's Republic of China.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Center for Advanced Low-dimension Materials, College of Material Science and Engineering , Donghua University , Shanghai 201620 , People's Republic of China.
Biomacromolecules. 2018 Mar 12;19(3):1065-1073. doi: 10.1021/acs.biomac.8b00190. Epub 2018 Feb 23.
Protein-based materials call for innovative processing techniques to integrate their unique biologically enabled functions with other materials of complementary features. Herein, we report the covalent protein layer-by-layer assembly via orthogonal "Tag-Catcher" reactions as a facile and robust approach to make entirely protein-based multilayers on a variety of substrates. Programmed assembly of native telechelic proteins not only endows the materials valuable stimuli-sensitive behaviors, but also unique properties unparalleled by any synthetic counterparts. As proof of concept, super uranyl-binding protein (SUP) is immobilized on silica gel by this method with tunable capacity and enhanced capability for uranyl sequestration. Not only is the capturing performance enhanced in the multilayer setup, it also confers resilience to recycling, allowing efficient harvest of uranyl with an average of ∼90% and ∼60% recovery rate in over 10 cycles from water and synthetic seawater, respectively. The approach is the first entirely protein-based multilayers covalently assembled by the layer-by-layer method. It provides a platform for immobilizing proteins with synergistic enhancement of function and resilience and expands the scope and capability of genetically encoded protein-based materials.
蛋白质基材料需要创新的加工技术，将其独特的生物功能与具有互补特性的其他材料集成在一起。在此，我们报告了通过正交的“Tag-Catcher”反应实现的共价蛋白质层层组装，这是一种在各种基底上制备完全基于蛋白质的多层膜的简便而强大的方法。天然端基蛋白质的程序化组装不仅赋予了材料有价值的刺激响应行为，而且还具有任何合成对应物都无法比拟的独特性能。作为概念验证，超铀结合蛋白（SUP）通过该方法固定在硅胶上，其结合铀的容量可调，且对铀的螯合能力增强。不仅在多层结构中提高了捕获性能，而且还使其具有可重复使用的弹性，从而能够从水中和人工海水中分别以约 90%和约 60%的平均回收率有效地回收铀，重复使用 10 次以上。该方法是首次通过层层组装方法制备完全基于蛋白质的多层膜，为协同增强功能和弹性的蛋白质固定提供了一个平台，并扩展了基于遗传编码蛋白质材料的范围和能力。
