Oh Nam Su, Joung Jae Yeon, Lee Ji Young, Kim Younghoon
R & D Center, Seoul Dairy Cooperative, Ansan, Kyunggi, South Korea.
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
PLoS One. 2018 Feb 14;13(2):e0192021. doi: 10.1371/journal.pone.0192021. eCollection 2018.
A total of 22 Lactobacillus strains, which were isolated from infant feces were evaluated for their probiotic potential along with resistance to low pH and bile salts. Eight isolates (L. reuteri 3M02 and 3M03, L. gasseri 4M13, 4R22, 5R01, 5R02, and 5R13, and L. rhamnosus 4B15) with high tolerance to acid and bile salts, and ability to adhere to the intestine were screened from 22 strains. Further, functional properties of 8 Lactobacillus strains, such as anti-oxidation, inhibition of α-glucosidase activity, cholesterol-lowering, and anti-inflammation were evaluated. The properties were strain-specific. Particularly, two strains of L. rhamnosus, 4B15 (4B15) and L. gasseri 4M13 (4M13) showed considerably higher anti-oxidation, inhibition of α-glucosidase activity, and cholesterol-lowering, and greater inhibition of nitric oxide production than other strains. Moreover, the two selected strains substantially inhibited the release of inflammatory mediators such as TNF-α, IL-6, IL-1β, and IL-10 stimulated the treatment of RAW 264.7 macrophages with LPS. In addition, whole genome sequencing and comparative genomic analysis of 4B15 and 4M13 indicated them as novel genomic strains. These results suggested that 4B15 and 4M13 showed the highest probiotic potential and have an impact on immune health by modulating pro-inflammatory cytokines.
对从婴儿粪便中分离出的总共22株乳酸杆菌菌株进行了益生菌潜力评估，同时评估了它们对低pH值和胆盐的耐受性。从22株菌株中筛选出8株对酸和胆盐具有高耐受性且能黏附肠道的菌株（罗伊氏乳杆菌3M02和3M03、加氏乳杆菌4M13、4R22、5R01、5R02和5R13以及鼠李糖乳杆菌4B15）。此外，还评估了这8株乳酸杆菌菌株的功能特性，如抗氧化、抑制α-葡萄糖苷酶活性、降低胆固醇和抗炎作用。这些特性具有菌株特异性。特别是，两株鼠李糖乳杆菌4B15（4B15）和加氏乳杆菌4M13（4M13）表现出比其他菌株更高的抗氧化、抑制α-葡萄糖苷酶活性和降低胆固醇的能力，以及对一氧化氮产生的更强抑制作用。此外，这两株选定的菌株显著抑制了脂多糖刺激RAW 264.7巨噬细胞处理后炎症介质如TNF-α、IL-6、IL-1β和IL-10的释放。此外，对4B15和4M13进行的全基因组测序和比较基因组分析表明它们是新的基因组菌株。这些结果表明，4B15和4M13表现出最高的益生菌潜力，并通过调节促炎细胞因子对免疫健康产生影响。
