Robbins Katherine S, Greenspan Phillip, Pegg Ronald B
Department of Food Science & Technology, College of Agricultural and Environmental Sciences, The University of Georgia, 100 Cedar Street, Athens, GA 30602-2610, USA.
Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens, GA 30602-2351, USA.
Food Chem. 2016 Dec 1;212:681-7. doi: 10.1016/j.foodchem.2016.06.018. Epub 2016 Jun 8.
Inflammation is linked to numerous chronic disease states. Phenolic compounds have attracted attention because a number of these compounds possess anti-inflammatory properties. A phenolic crude extract was prepared from pecans and separated by Sephadex LH-20 column chromatography into low- and high-molecular-weight (LMW/HMW) fractions. Anti-inflammatory properties of these fractions were assessed in LPS-stimulated RAW 264.7 murine macrophage cells. NO and reactive oxygen species (ROS) production was monitored after 3 different experimental protocols: (1) pre-treatment with Escherichia coli O111:B4 lipopolysaccharide (LPS); (2) pre-treatment with a pecan crude extract and its fractions; and (3) co-incubation of LPS with a pecan crude extract and its fractions. The LMW fraction displayed a dose-dependent decrease in NO production and a significant decrease from the LPS control in ROS production when cells were either co-incubated with or pre-treated with LPS. The phenolics were characterized by HPLC to help identify those responsible for the observed effect.
炎症与多种慢性疾病状态相关。酚类化合物因其多种此类化合物具有抗炎特性而受到关注。从山核桃中制备了酚类粗提物，并通过葡聚糖LH - 20柱色谱法分离成低分子量和高分子量（LMW/HMW）组分。在脂多糖（LPS）刺激的RAW 264.7小鼠巨噬细胞中评估了这些组分的抗炎特性。在3种不同的实验方案后监测一氧化氮（NO）和活性氧（ROS）的产生：（1）用大肠杆菌O111:B4脂多糖（LPS）预处理；（2）用山核桃粗提物及其组分预处理；（3）将LPS与山核桃粗提物及其组分共同孵育。当细胞与LPS共同孵育或用LPS预处理时，低分子量组分显示出NO产生的剂量依赖性降低以及ROS产生相对于LPS对照组的显著降低。通过高效液相色谱法（HPLC）对酚类进行了表征，以帮助鉴定导致观察到的效果的那些酚类。
