Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa.
Probiotics Antimicrob Proteins. 2018 Mar;10(1):89-97. doi: 10.1007/s12602-017-9307-5.
Human feces were streaked onto MRS Agar adjusted to pH 2.5, 3.0, and 6.4, respectively, and medium supplemented with 1.0% (w/v) bile salts. Two aciduric strains, identified as Lactobacillus reuteri HFI-LD5 and Lactobacillus rhamnosus HFI-K2 (based on 16S rDNA and recA sequences), were non-hemolytic and did not hydrolyze mucin. The surface of Lactobacillus reuteri HFI-LD5 cells has a weak negative charge, whereas Lactobacillus rhamnosus HFI-K2 has acidic and basic properties, and produces exopolysaccharides (EPS). None of the strains produce bacteriocins. Both strains are resistant to several antibiotics, including sulfamethoxazole-trimethoprim and sulphonamides. The ability of Lactobacillus reuteri HFI-LD5 and Lactobacillus rhamnosus HFI-K2 to grow at pH 2.5 suggests that they will survive passage through the stomach. EPS production may assist in binding to intestinal mucus, especially in the small intestinal tract, protect epithelial cells, and stimulate the immune system. Lactobacillus reuteri HFI-LD5 and Lactobacillus rhamnosus HFI-K2 may be used as probiotics, especially in the treatment of small intestinal bacterial overgrowth (SIBO).
人粪便分别涂抹在 pH 值分别为 2.5、3.0 和 6.4 的 MRS 琼脂和添加 1.0%（w/v）胆盐的培养基上。两种耐酸菌株，鉴定为雷氏乳杆菌 HFI-LD5 和鼠李糖乳杆菌 HFI-K2（基于 16S rDNA 和 recA 序列），均不溶血且不能水解粘蛋白。雷氏乳杆菌 HFI-LD5 细胞表面带微弱负电荷，而鼠李糖乳杆菌 HFI-K2 具有酸性和碱性性质，并产生胞外多糖（EPS）。两种菌株均不产生细菌素。两种菌株均对包括磺胺甲噁唑-甲氧苄啶和磺胺类药物在内的多种抗生素具有抗性。雷氏乳杆菌 HFI-LD5 和鼠李糖乳杆菌 HFI-K2 在 pH 值 2.5 下生长的能力表明它们将能够在胃中存活。EPS 的产生可能有助于与肠道粘液结合，特别是在小肠中，保护上皮细胞并刺激免疫系统。雷氏乳杆菌 HFI-LD5 和鼠李糖乳杆菌 HFI-K2 可以用作益生菌，特别是在治疗小肠细菌过度生长（SIBO）时。
