Institut für Biochemie, Freie Universität Berlin, Berlin, Germany
J Cell Biol. 2018 Mar 5;217(3):809-811. doi: 10.1083/jcb.201802025. Epub 2018 Feb 14.
Secondary antibodies are everyday reagents in biomedical research that are generated in animals. In this issue, Pleiner et al. (2018. https://doi.org/10.1083/jcb.201709115) describe several single domain antibody fragments against antibodies from mouse and rabbit, so-called nanobodies that are easily produced recombinantly, and characterize their use in Western blotting, enzyme-linked immunosorbent assay, and immunofluorescence assays.
二抗是生物医学研究中常用的试剂，通常在动物体内产生。在本期中，Pleiner 等人（2018 年，https://doi.org/10.1083/jcb.201709115）描述了几种针对小鼠和兔抗体的单域抗体片段，即所谓的纳米抗体，它们可以通过重组方式轻松产生，并描述了它们在 Western blot、酶联免疫吸附测定和免疫荧光检测中的应用。
