Hashemifar Iman, Masjedian Jazi Faramarz, Yadegar Abbas, Amirmozafari Nour
. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
. Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Med J Islam Repub Iran. 2017 Sep 7;31:57. doi: 10.14196/mjiri.31.57. eCollection 2017.
Human brucellosis is a zoonotic disease caused by Brucella melitensis, , and . causes a chronic disease, which subverts the immune defense system of their hosts. In this study, the prevalence of an important virulence determinant, , which can modulate immune response, was determined in human isolates. Polymerase chain reaction (PCR) assay was standardized and applied to 37 isolates obtained from patient's specimens. Primers for gene were designed and evaluated using bioinformatic tools. DNA sequencing was performed for further verification. In the 37 isolates (31 melitensis and 6 abortus), 32 (86.4%) carried gene. Presence of gene in most isolates indicates the high prevalence of this gene among Iranian isolates and emphasizes its role in pathogenicity of this organism.
人类布鲁氏菌病是一种由羊布鲁氏菌引起的人畜共患病。羊布鲁氏菌会引发一种慢性疾病，它会破坏宿主的免疫防御系统。在本研究中，对一种可调节免疫反应的重要毒力决定因素——[具体因素未给出]在人类分离株中的流行情况进行了测定。聚合酶链反应（PCR）检测方法得到了标准化，并应用于从患者标本中获得的37株分离株。使用生物信息学工具设计并评估了用于[具体基因未给出]基因的引物。进行了DNA测序以作进一步验证。在这37株分离株（31株羊布鲁氏菌和6株流产布鲁氏菌）中，32株（86.4%）携带[具体基因未给出]基因。大多数分离株中存在[具体基因未给出]基因，这表明该基因在伊朗分离株中具有较高的流行率，并强调了其在该生物体致病性中的作用。
