Hashemifar Iman, Yadegar Abbas, Jazi Faramarz Masjedian, Amirmozafari Nour
Microbiology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Microb Pathog. 2017 Apr;105:334-339. doi: 10.1016/j.micpath.2017.03.007. Epub 2017 Mar 8.
Molecular prevalence of nine putative virulence factors in two more prevalent Brucella species in Iranian patients and livestock was investigated. During five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of Iran. All samples were cultured in blood culture media and subcultured into Brucella agar medium. Nine primer pairs were designed for detection of VirB2, VirB5, VceC, BtpA, BtpB, PrpA, BetB, BPE275 and BSPB virulence factors using PCR and sequence analysis. Totally, 68 Brucella isolates including 60 B. melitensis and 8 B. abortus were isolated from the human and animal specimens examined. Approximately, all B. melitensis and B. abortus strains were positive (100%) regarding btpA, btpB, virB5, vceC, bpe275, bspB, and virB2 genes except for prpA and betB that were detected in 86% and 97% of the strains, respectively. Significant relationships were found between the presence of prpA and human B. melitensis isolates (P = 0.04), and also between the presence of betB and human isolates of B. abortus (P = 0.03). In conclusion, our results revealed that Iranian Brucella strains, regardless of human or animal sources, are extremely virulent due to high prevalence of virulence attributes in almost all strains studied.
对伊朗患者和家畜中两种更常见布鲁氏菌物种的九种假定毒力因子的分子流行情况进行了调查。在五年（2010 - 2015年）期间，从伊朗三个地理区域收集了120份人类和动物标本。所有样本均在血培养基中培养，并转种至布鲁氏菌琼脂培养基。设计了九对引物，用于通过聚合酶链反应（PCR）和序列分析检测VirB2、VirB5、VceC、BtpA、BtpB、PrpA、BetB、BPE275和BSPB毒力因子。总共，从所检查的人类和动物标本中分离出68株布鲁氏菌，其中包括60株羊种布鲁氏菌和8株牛种布鲁氏菌。大约，所有羊种布鲁氏菌和牛种布鲁氏菌菌株的btpA、btpB、virB5、vceC、bpe275、bspB和virB2基因均呈阳性（100%），除了PrpA和BetB，分别在86%和97%的菌株中检测到。发现PrpA的存在与人类羊种布鲁氏菌分离株之间存在显著关系（P = 0.04），BetB的存在与人类牛种布鲁氏菌分离株之间也存在显著关系（P = 0.03）。总之，我们的结果表明，伊朗的布鲁氏菌菌株，无论来源是人还是动物，由于在所研究的几乎所有菌株中毒力属性的高流行率，都具有极强的毒力。
