Eulji University, Department of Senior Healthcare, Daejeon, Republic of Korea.
Korea Institute of Radiological and Medical Science, Division of Medical Radiation Equipment, Nowon-, Republic of Korea.
J Biomed Opt. 2018 Feb;23(2):1-13. doi: 10.1117/1.JBO.23.2.026003.
Optical imaging techniques are widely used for in vivo preclinical studies, and it is well known that the Geant4 Application for Emission Tomography (GATE) can be employed for the Monte Carlo (MC) modeling of light transport inside heterogeneous tissues. However, the GATE MC toolkit is limited in that it does not yet include optical lens implementation, even though this is required for a more realistic optical imaging simulation. We describe our implementation of a biconvex lens into the GATE MC toolkit to improve both the sensitivity and spatial resolution for optical imaging simulation. The lens implemented into the GATE was validated against the ZEMAX optical simulation using an US air force 1951 resolution target. The ray diagrams and the charge-coupled device images of the GATE optical simulation agreed with the ZEMAX optical simulation results. In conclusion, the use of a lens on the GATE optical simulation could improve the image quality of bioluminescence and fluorescence significantly as compared with pinhole optics.
光学成象技术广泛应用于活体临床前研究，众所周知，Geant4 发射断层成像应用（GATE）可用于模拟非均相组织内的光传输的蒙特卡罗（MC）建模。然而，GATE MC 工具包存在局限性，因为它尚未包括光学透镜的实现，尽管对于更现实的光学成象模拟这是必需的。我们描述了将双凸透镜实施到 GATE MC 工具包中的情况，以提高光学成象模拟的灵敏度和空间分辨率。利用美国空军 1951 分辨率靶标对 ZEMAX 光学模拟进行验证，将实施到 GATE 中的透镜与 ZEMAX 光学模拟结果进行了比较。总之，与针孔光学相比，在 GATE 光学模拟中使用透镜可以显著提高生物发光和荧光的图像质量。
