Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
Max Planck Institute of Biophysics, Frankfurt am Main, Germany.
Elife. 2018 Feb 15;7:e33322. doi: 10.7554/eLife.33322.
In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells with either TTX or bicucculline for 2 hr to induce the process of up- or down-scaling, respectively. During the activity manipulation we metabolically labeled newly synthesized proteins using BONCAT. We identified 168 newly synthesized proteins that exhibited significant changes in expression. To obtain a temporal trajectory of the response, we compared the proteins synthesized within 2 hr or 24 hr of the activity manipulation. Surprisingly, there was little overlap in the significantly regulated newly synthesized proteins identified in the early- and integrated late response datasets. There was, however, overlap in the functional categories that are modulated early and late. These data indicate that within protein function groups, different proteomic choices can be made to effect early and late homeostatic responses that detect the duration and polarity of the activity manipulation.
在中枢突触的稳态缩放中，检测与设定点的偏差、检测偏差持续时间以及实施细胞反应的细胞机制的深度和广度尚不清楚。为了了解时间依赖的缩放动态，我们用 TTX 或双环己酰亚胺分别处理培养的大鼠海马细胞 2 小时，分别诱导上或下缩放过程。在活动处理过程中，我们使用 BONCAT 对新合成的蛋白质进行代谢标记。我们鉴定了 168 种表达发生显著变化的新合成蛋白质。为了获得反应的时间轨迹，我们比较了在活动处理后 2 小时或 24 小时内合成的蛋白质。令人惊讶的是，在早期和综合晚期反应数据集中鉴定的显著调节的新合成蛋白质之间几乎没有重叠。然而，在早期和晚期调节的功能类别之间存在重叠。这些数据表明，在蛋白质功能组内，可以选择不同的蛋白质组学来实现早期和晚期的稳态反应，以检测活动处理的持续时间和极性。
