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基于从抗酸染色载玻片提取的 DNA 进行 spoligotyping 获得的尼日利亚流行的结核分枝杆菌复合群基因型。

Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl-Neelsen stained slides extracted DNA.

机构信息

Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Fundacio Institut d'Investigació Germans Trias i Pujol. Universitat Autònoma de Barcelona. CIBER Enfermedades Respiratorias, Badalona, Spain.

Institut for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris Sud, Université Paris-Saclay, Gif-sur-Yvette, France.

出版信息

PLoS Negl Trop Dis. 2018 Feb 15;12(2):e0006242. doi: 10.1371/journal.pntd.0006242. eCollection 2018 Feb.

DOI:10.1371/journal.pntd.0006242
PMID:29447161
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5831734/
Abstract

METHODS

All State TB control programmes in Nigeria were requested to submit 25-50 smear-positive Ziehl-Neelsen (ZN) stained slides for screening during 2013-2014. DNA was extracted from 929 slides for spoligotyping and drug-resistance analysis using microbead-based flow-cytometry suspension arrays.

RESULTS

Spoligotyping results were obtained for 549 (59.1%) of 929 samples. Lineage 4 Cameroon sublineage (L4.6.2) represented half of the patterns, Mycobacterium africanum (L5 and L6) represented one fifth of the patterns, and all other lineages, including other L4 sublineages, represented one third of the patterns. Sublineage L4.6.2 was mostly identified in the north of the country whereas L5 was mostly observed in the south and L6 was scattered. The spatial distribution of genotypes had genetic geographic gradients. We did not obtain results enabling the detection of drug-resistance mutations.

CONCLUSION/SIGNIFICANCE: We present the first national snapshot of the M. tuberculosis spoligotypes circulating in Nigeria based on ZN slides. Spoligotyping data can be obtained in a rapid and high-throughput manner with DNA extracted from ZN-stained slides, which may potentially improve our understanding of the genetic epidemiology of TB.

摘要

方法

尼日利亚所有州立结核病控制项目均被要求在 2013-2014 年期间提交 25-50 张 Ziehl-Neelsen（ZN）染色阳性的痰涂片进行筛查。从 929 张涂片提取 DNA，采用微珠流式细胞术悬浮芯片进行 spoligotyping 和耐药性分析。

结果

549 份（929 份的 59.1%）样本获得 spoligotyping 结果。谱系 4 喀麦隆亚谱系（L4.6.2）占模式的一半，非洲分枝杆菌（L5 和 L6）占模式的五分之一，其他所有谱系，包括其他 L4 亚谱系，占模式的三分之一。L4.6.2 亚谱系主要在该国北部发现，而 L5 主要在南部观察到，L6 则分布广泛。基因型的空间分布存在遗传地理梯度。我们没有获得能够检测耐药突变的结果。

结论/意义：我们根据 ZN 幻灯片呈现了尼日利亚结核分枝杆菌 spoligotype 首次全国性快照。ZN 染色涂片提取的 DNA 可以快速高通量地获得 spoligotyping 数据，这可能有助于我们更好地了解结核病的遗传流行病学。

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