a Department of Applied Chemistry , National Chiao Tung University , Hsinchu , Taiwan.
Artif Cells Nanomed Biotechnol. 2018;46(sup1):841-851. doi: 10.1080/21691401.2018.1438449. Epub 2018 Feb 15.
Escherichia coli O157:H7 is a pathogen, which can generate Shiga-like toxins (SLTs) and cause hemolytic-uremic syndrome. Foodborne illness outbreaks caused by E. coli O157:H7 have become a global issue. Since SLTs are quite toxic, effective medicines that can reduce the damage caused by SLTs should be explored. SLTs consist of a single A and five B subunits, which can inhibit ribosome activity for protein synthesis and bind with the cell membrane of host cells, respectively. Pigeon ovalbumin (POA), i.e. a glycoprotein, is abundant in pigeon egg white (PEW) proteins. The structure of POA contains Gal-α(1→4)-Gal-β(1→4)-GlcNAc ligands, which have binding affinity toward the B subunit in SLT type-1 (SLT-1B). POA immobilized gold nanoparticles (POA-Au NPs) can be generated by reacting PEW proteins with aqueous tetrachloroauric acid in one-pot. The generated POA-Au NPs have been demonstrated to have selective trapping-capacity toward SLT-1B previously. Herein, we explore that POA-Au NPs can be used as protective agents to neutralize the toxicity of SLT-1 in SLT-1-infected model cells. The results show that the cells can be completely rescued when a sufficient amount of POA-Au NPs is used to treat the SLT-1-infected cells within 1 h.
产志贺样毒素大肠杆菌 O157:H7 是一种病原体，可产生志贺样毒素（SLTs）并引起溶血性尿毒综合征。由产志贺样毒素大肠杆菌 O157:H7 引起的食源性疾病爆发已成为全球性问题。由于 SLTs 毒性很强，应该探索能够减少 SLTs 造成损害的有效药物。SLTs 由一个 A 亚基和五个 B 亚基组成，可分别抑制核糖体活性以阻止蛋白质合成，并与宿主细胞的细胞膜结合。鸽子卵清蛋白（POA），即糖蛋白，在鸽子蛋清（PEW）蛋白中含量丰富。POA 的结构含有 Gal-α(1→4)-Gal-β(1→4)-GlcNAc 配体，与 SLT-1 型（SLT-1B）中的 B 亚基具有结合亲和力。POA 与水合四氯金酸在一锅法中反应可生成 POA 固定化金纳米粒子（POA-Au NPs）。先前已经证明，生成的 POA-Au NPs 对 SLT-1B 具有选择性捕获能力。在此，我们探索了 POA-Au NPs 可作为保护剂用于中和 SLT-1 感染模型细胞中 SLT-1 的毒性。结果表明，当使用足够量的 POA-Au NPs 在 1 小时内处理 SLT-1 感染的细胞时，细胞可以被完全挽救。
