Gong Ping, Shi Bingwei, Wang Juan, Cao Peixia, Diao Zhenyu, Wang Yuji, Hu Yali, Li Shuping
a Department of Obstetrics and Gynecology , Changzhou Hospital of Traditional Chinese Medicine, Nanjing University of Chinese Medicine , Changzhou , China.
b Department of Obstetrics and Gynecology , Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine , Nanjing , China.
Gynecol Endocrinol. 2018 Aug;34(8):709-714. doi: 10.1080/09513590.2018.1428301. Epub 2018 Feb 15.
This study aimed to investigate the Th1/Th2 cells in peripheral blood of PCOS patients, and assess the potential correlation between Th1/Th2 imbalance and obesity.
Thirty-nine PCOS patients and 23 age-matched controls were enrolled. The PBMCs were obtained before pharmacological intervention in women with or without PCOS. The profiles of Th1 (IFN-γ) and Th2 (IL-4) cytokines of CD3CD T lymphocyte subsets were analyzed by flow cytometry. Plasma sex hormones including E, T, FSH, LH, and FINS, FPG were measured, together with BMI, WC, LH/FSH, E/T and HOMA-IR index being calculated. Association between Th1/Th2 imbalance and BMI, WC were evaluated.
The proportion of Th1 cells and Th1/Th2 ratio were significantly higher in PCOS patients than those in controls, accompanied by elevated T, LH, LH/FSH, FINS, HOMA-IR index and reduced E/T. The Th1/Th2 ratio was increased when BMI and WC were enhanced in PCOS. Moreover, the significant difference of Th1/Th2 ratio was observed between WC subgroups of PCOS.
It is concluded that Th1 type immunity is predominant in systemic immunization of PCOS patients. Th1/Th2 immune imbalance is connected with obesity, especially abdominal obesity, and may be one of the underlying mechanism for the pathogenesis of PCOS.
本研究旨在调查多囊卵巢综合征（PCOS）患者外周血中的Th1/Th2细胞，并评估Th1/Th2失衡与肥胖之间的潜在相关性。
招募39例PCOS患者和23例年龄匹配的对照。在有或无PCOS的女性进行药物干预前获取外周血单个核细胞（PBMC）。通过流式细胞术分析CD3⁺CD⁺ T淋巴细胞亚群的Th1（IFN-γ）和Th2（IL-4）细胞因子谱。检测血浆性激素包括雌二醇（E）、睾酮（T）、促卵泡生成素（FSH）、促黄体生成素（LH）以及空腹胰岛素（FINS）、空腹血糖（FPG），并计算体重指数（BMI）、腰围（WC）、LH/FSH、E/T和胰岛素抵抗稳态模型评估指数（HOMA-IR）。评估Th1/Th2失衡与BMI、WC之间的关联。
PCOS患者中Th1细胞比例和Th1/Th2比值显著高于对照组，同时T、LH、LH/FSH、FINS、HOMA-IR指数升高，E/T降低。在PCOS患者中，随着BMI和WC增加，Th1/Th2比值升高。此外，PCOS患者WC亚组间Th1/Th2比值存在显著差异。
得出结论，Th1型免疫在PCOS患者的全身免疫中占主导。Th1/Th2免疫失衡与肥胖尤其是腹型肥胖相关，可能是PCOS发病机制的潜在机制之一。
