Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai 400061, India.
Division of Fish Nutrition and Feed Technology, ICAR-Central Institute of Fisheries Education, Mumbai 400061, India.
Int J Biol Macromol. 2018 Jun;112:1093-1103. doi: 10.1016/j.ijbiomac.2018.02.067. Epub 2018 Feb 13.
Chitosan nanoparticles (CNPs) have been proven considerable delivery agents due to their remarkable physicochemical properties. Present study reports the fabrication of CNPs by ionic gelation process and their characterization by different approaches. The constructed nanoparticles were successfully conjugated with eurycomanone with significant entrapment efficiency. Particle size of chitosan and chitosan conjugated eurycomanone nanoparticles were 126.2nm and 130nm respectively. Scanning electron microscopy showed that the particles were spherical in shape and well dispersed. Cross-linking between CNPs and eurycomanone (CENPs) were confirmed by Fourier-transform infrared (FTIR) spectroscopy. Fluorescent nanoparticles were prepared by using Rhodamine-6G dye, characterised by SEM and confirmed for conjugation by FTIR. Biodistribution of CENPs showed the presence of fluorescent nanoparticles in liver, kidney, testes and brain of C. magur. The toxicity of CENPs was evaluated by comparing the histological sections of catfish testes collected from treated and control group. No signs of toxicity were seen in testes after the delivery of CENPs. Molecular docking study revealed high spontaneous binding ability of chitosan with eurycomanone and aromatase enzyme. The study reports that CNPs can act as a stabilizing agent for eurycomanone formulation and could be a promising approach to increase the reproductive performance of the fishes.
壳聚糖纳米粒子（CNPs）因其显著的物理化学性质而被证明是一种很有前途的药物递送载体。本研究报告了通过离子凝胶化法制备 CNPs 及其通过不同方法进行的表征。构建的纳米粒子成功地与原人参二醇缀合，具有显著的包封效率。壳聚糖和壳聚糖缀合原人参二醇纳米粒子的粒径分别为 126.2nm 和 130nm。扫描电子显微镜显示，这些粒子呈球形，分散良好。傅里叶变换红外（FTIR）光谱证实了 CNPs 与原人参二醇（CENPs）之间的交联。通过使用罗丹明 6G 染料制备荧光纳米粒子，通过 SEM 进行表征，并通过 FTIR 确认缀合。CENPs 的生物分布显示，荧光纳米粒子存在于 magur 鲤鱼的肝、肾、睾丸和脑中。通过比较从处理组和对照组收集的鲶鱼睾丸的组织切片来评估 CENPs 的毒性。在递送 CENPs 后，睾丸没有出现毒性迹象。分子对接研究表明，壳聚糖与原人参二醇和芳香酶具有很高的自发结合能力。该研究报告称，CNPs 可以作为原人参二醇制剂的稳定剂，并可能成为提高鱼类繁殖性能的一种有前途的方法。
