Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100, Murcia, Spain.
Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100, Murcia, Spain; Fish Nutrition & Immunobiology Group, Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208, Porto, Portugal.
Fish Shellfish Immunol. 2018 Apr;75:291-294. doi: 10.1016/j.fsi.2018.02.017. Epub 2018 Feb 12.
Skin lesions contribute to significant rates of morbidity and mortality in fish because they permit the entry of pathogens. Different seric immune parameters (IgM level, protease, antiprotease, peroxidase and lysozyme) and bactericidal activity (against Vibrio harveyi and Photobacterium damsealae) of gilthead seabream (Sparus aurata L.) specimens were evaluated after experimentally wounding fish in two body locations (above or below the lateral line). The results demonstrate that the level of several immune parameters present in fish serum (IgM, proteases, peroxidase and bactericidal activity) showed statistically significant variations depending on the site of the skin wound and the time post-wounding (from 0 to 7 days). However, other parameters (such as antiproteases or lysozyme) remained unaltered during the experiment and did not differs from the values recorded on control fish (non-wounded). The highest activities recorded coincided with the inflammatory healing phase. Moreover, many more significant variations were observed in fish wounded below the lateral line than in those wounded above the lateral line. The present results demonstrate the importance of skin integrity in the maintenance of fish body homeostasis.
皮肤损伤会导致鱼类发病率和死亡率的显著上升，因为它们允许病原体进入。本研究评估了在斜线下方和上方两个不同的身体部位对实验性刺伤的真鲷（Sparus aurata L.）血清中的几种血清免疫参数（IgM 水平、蛋白酶、抗蛋白酶、过氧化物酶和溶菌酶）和杀菌活性（针对哈维氏弧菌和美人鱼发光杆菌）。结果表明，鱼类血清中存在的几种免疫参数（IgM、蛋白酶、过氧化物酶和杀菌活性）的水平根据皮肤伤口的位置和受伤后的时间（从 0 到 7 天）呈现出统计学上的显著变化。然而，其他参数（如抗蛋白酶或溶菌酶）在实验过程中保持不变，与对照鱼（未受伤）记录的值没有差异。记录到的最高活性与炎症愈合阶段相对应。此外，在斜线下方受伤的鱼比在斜线上方受伤的鱼观察到更多的显著变化。本研究结果表明，皮肤完整性对维持鱼类体内平衡具有重要意义。
