Translational Regenerative Medicine Laboratory, Kolling Institute, Royal North Shore Hospital, Sydney, Australia.
Northern Clinical School, Faculty of Medicine, The University of Sydney, Sydney, Australia.
Sci Rep. 2018 Feb 15;8(1):3101. doi: 10.1038/s41598-018-21387-w.
Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous studies have identified, but not quantified, a small number of chemokines associated with RBCs. We isolated RBCs from healthy volunteers and quantified of a panel of 48 cytokines, chemokines, and growth factors in the lysate, cytosol, and conditioned media of these cells using Luminex technology. This analysis revealed that, after correcting for white blood cell and platelet contamination, 46 cytokines were detected in RBC lysates, and the median concentration in RBCs was 12-fold higher than in the plasma. In addition, extensive washing of RBCs, such as that performed in proteomics analyses or prior to some RBC transfusions, significantly attenuated the release of six cytokines following incubation at 37 °C. This supports the hypothesis that, alongside its gas exchange function, RBCs play a role in cytokine signalling. This discovery may help supplement disease biomarker research and may shed light on adverse inflammatory processes that can follow RBC transfusion.
红细胞（RBCs）已被证明会影响免疫功能，并在输血后引发炎症反应。洗涤红细胞的输注可以显著减少不良反应，但是介导这些作用的可溶性因子尚未确定。先前的研究已经确定了与 RBC 相关的，但没有定量的少数趋化因子。我们从健康志愿者中分离 RBC，并使用 Luminex 技术在这些细胞的裂解物、胞浆和条件培养基中定量分析了 48 种细胞因子、趋化因子和生长因子。这项分析表明，在纠正白细胞和血小板污染后，在 RBC 裂解物中检测到 46 种细胞因子，并且 RBC 中的中位数浓度比血浆高 12 倍。此外，对 RBC 进行广泛洗涤，如在蛋白质组学分析中或在某些 RBC 输血之前进行的洗涤，可显著减少在 37°C 孵育后六种细胞因子的释放。这支持了 RBC 除了其气体交换功能外，还在细胞因子信号转导中发挥作用的假说。这一发现可能有助于补充疾病生物标志物研究，并阐明可能紧随 RBC 输血而来的不良炎症过程。
