Department of Genetics, Universidade Federal do Parana, Av. Coronel Francisco Heráclito dos Santos, 210. CEP, 81531-970, Curitiba, PR, Brazil.
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky, 40536-0596, USA.
Sci Rep. 2018 Feb 15;8(1):3122. doi: 10.1038/s41598-018-21400-2.
Microorganisms associated with plants are highly diverse and can produce a large number of secondary metabolites, with antimicrobial, anti-parasitic and cytotoxic activities. We are particularly interested in exploring endophytes from medicinal plants found in the Pantanal, a unique and widely unexplored wetland in Brazil. In a bio-prospecting study, strains LGMF1213 and LGMF1215 were isolated as endophytes from Vochysia divergens, and by morphological and molecular phylogenetic analyses were characterized as Phaeophleospora vochysiae sp. nov. The chemical assessment of this species reveals three major compounds with high biological activity, cercoscosporin (1), isocercosporin (2) and the new compound 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone (3). Besides the isolation of P. vochysiae as endophyte, the production of cercosporin compounds suggest that under specific conditions this species causes leaf spots, and may turn into a pathogen, since leaf spots are commonly caused by species of Cercospora that produce related compounds. In addition, the new compound 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone showed considerable antimicrobial activity and low cytotoxicity, which needs further exploration.
与植物相关的微生物种类多样，能产生大量具有抗菌、抗寄生虫和细胞毒性的次生代谢产物。我们特别感兴趣的是探索在潘塔纳尔湿地（巴西独特且尚未广泛探索的湿地）中发现的药用植物内生菌。在生物勘探研究中，从 Vochysia divergens 中分离出内生菌 LGMF1213 和 LGMF1215，通过形态学和分子系统发育分析，将其鉴定为 Phaeophleospora vochysiae sp. nov. 该物种的化学评估揭示了三种具有高生物活性的主要化合物，即 cercoscosporin (1)、isocercosporin (2) 和新化合物 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone (3)。除了分离出 Phaeophleospora vochysiae 作为内生菌外， cercoscosporin 化合物的产生表明在特定条件下，该物种会引起叶斑病，并且可能会变成病原体，因为叶斑病通常是由产生相关化合物的 Cercospora 物种引起的。此外，新化合物 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone 表现出相当强的抗菌活性和低细胞毒性，需要进一步探索。
