Matson P L, Yovich J M, Junk S, Bootsma B, Yovich J L
J In Vitro Fert Embryo Transf. 1986 Aug;3(4):227-31. doi: 10.1007/BF01132809.
Two groups of women were studied in whom a proportion of follicles had either ovulated spontaneously (7 women) or ruptured during manipulation at laparoscopy (30 women), and oocytes were recovered from the pouch of Douglas. There were no significant differences in the fertilization rates of oocytes collected in the pouch of Douglas from ovulated follicles, compared with those from the remaining intact follicles [15/20 (75%) vs 14/20 (70%)]. Also, there was no significant difference between the fertilization rate of oocytes from follicles ruptured at the time of oocyte collection and that of oocytes from intact follicles [25/38 (66%) vs 101/140 (72%)]. One woman became pregnant, following the transfer of four four-cell embryos, all derived from spontaneously ovulated oocytes found in the pouch of Douglas. She gave birth to a baby girl. The present study has shown that oocytes may still be retrieved from the pouch of Douglas, despite follicle dispersal; these oocytes can be fertilized; and the embryos derived from ovulated oocytes recovered from the pouch of Douglas may generate an ongoing pregnancy following in vitro fertilization and embryo transfer.
对两组女性进行了研究，其中一部分卵泡要么自然排卵（7名女性），要么在腹腔镜操作过程中破裂（30名女性），并从Douglas窝中回收卵母细胞。与从其余完整卵泡中收集的卵母细胞相比，从Douglas窝中收集的来自已排卵卵泡的卵母细胞的受精率没有显著差异[15/20（75%）对14/20（70%）]。此外，在卵母细胞采集时破裂的卵泡的卵母细胞受精率与完整卵泡的卵母细胞受精率之间也没有显著差异[25/38（66%）对101/140（72%）]。一名女性在移植了四个四细胞胚胎后怀孕，所有胚胎均来自Douglas窝中发现的自然排卵的卵母细胞。她生下了一个女婴。本研究表明，尽管卵泡已分散，但仍可从Douglas窝中获取卵母细胞；这些卵母细胞可以受精；并且从Douglas窝中回收的来自已排卵卵母细胞的胚胎在体外受精和胚胎移植后可能会实现持续妊娠。
