Xiao Lifu, Parchur Abdul K, Gilbertson Timothy A, Zhou Anhong
Department of Biological Engineering, Utah State University, Logan, Utah 84322-4105, U.S.A.
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, 46556, USA.
Anal Methods. 2018;10(1):22-29. doi: 10.1039/C7AY02039B. Epub 2017 Oct 18.
G-protein-coupled receptor 120 (GPR120), as a member of the rhodopsin family of G-protein-coupled receptors, has been shown to function as a sensor for dietary fat in the gustatory and digestive systems. Its specific role in the chemoreception of fatty acids, which is thought to be crucial in understanding the mechanism surrounding the control of fat intake and, accordingly, in the treatment of obesity, remains unclear. Here we report a novel surface-enhanced Raman spectroscopy (SERS)-fluorescence bimodal microscopic technique for detection and imaging of GPR120 in single living cells. CaMoO:Eu@AuNR hybrid nanoparticles are synthesized and characterized as imaging probes. Biocompatibility and imaging capability of the probes are investigated using a model HEK293 cell line with an inducible GPR120 gene transfection. Cellular distribution of GPR120 is visualized by single-cell SERS and fluorescence imaging. A dose-dependent GPR120 response to linoleic acid treatment is revealed by SERS.
G蛋白偶联受体120（GPR120）作为G蛋白偶联受体视紫红质家族的一员，已被证明在味觉和消化系统中作为膳食脂肪的传感器发挥作用。其在脂肪酸化学感受中的具体作用尚不清楚，而脂肪酸化学感受被认为对于理解脂肪摄入控制机制以及相应地治疗肥胖症至关重要。在此，我们报告了一种用于在单个活细胞中检测和成像GPR120的新型表面增强拉曼光谱（SERS）-荧光双峰显微技术。合成了CaMoO:Eu@AuNR混合纳米颗粒并将其表征为成像探针。使用具有可诱导GPR1基因转染的模型HEK293细胞系研究了探针的生物相容性和成像能力。通过单细胞SERS和荧光成像可视化GPR120的细胞分布。SERS揭示了GPR120对亚油酸处理的剂量依赖性反应。 120
原文中“GPR1基因转染”疑似有误，应为“GPR120基因转染”，译文已按此修正。
