Pilap Warayutt, Thanonkeo Sudarat, Klanrit Preekamol, Thanonkeo Pornthap
1Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002 Thailand.
2Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, 44150 Thailand.
3 Biotech. 2018 Feb;8(2):126. doi: 10.1007/s13205-018-1161-y. Epub 2018 Feb 13.
In this work, the newly isolated thermotolerant DBKKUY-103 exhibited a high ethanol fermentation efficiency at high temperatures using sweet sorghum juice (SSJ). The highest ethanol concentrations and productivities achieved under the optimum conditions using thermotolerant DBKKUY-103 were 85.16 g/l and 1.42 g/l.h at 37 °C and 83.46 g/l and 1.39 g/l.h at 40 °C, respectively. The expression levels of genes during ethanol fermentation at 40 °C were evaluated and the results found that the transcriptional levels of the , , , , , , , and genes were up-regulated compared with those at 30 °C, suggesting that the high growth and high ethanol production efficiencies of DBKKUY-103 during high-temperature ethanol production associated with the genes involved in DNA repair and ATP production.
在本研究中，新分离的耐热菌株DBKKUY-103在高温下利用甜高粱汁（SSJ）表现出较高的乙醇发酵效率。使用耐热菌株DBKKUY-103在最佳条件下，在37℃时达到的最高乙醇浓度和生产率分别为85.16 g/l和1.42 g/l·h，在40℃时分别为83.46 g/l和1.39 g/l·h。对40℃乙醇发酵过程中基因的表达水平进行了评估，结果发现，与30℃相比，、、、、、、和基因的转录水平上调，这表明DBKKUY-103在高温乙醇生产过程中的高生长和高乙醇生产效率与参与DNA修复和ATP生成的基因有关。
