The potential of the newly isolated thermotolerant for high-temperature ethanol production using sweet sorghum juice.

In this work, the newly isolated thermotolerant DBKKUY-103 exhibited a high ethanol fermentation efficiency at high temperatures using sweet sorghum juice (SSJ). The highest ethanol concentrations and productivities achieved under the optimum conditions using thermotolerant DBKKUY-103 were 85.16 g/l and 1.42 g/l.h at 37 °C and 83.46 g/l and 1.39 g/l.h at 40 °C, respectively. The expression levels of genes during ethanol fermentation at 40 °C were evaluated and the results found that the transcriptional levels of the , , , , , , , and genes were up-regulated compared with those at 30 °C, suggesting that the high growth and high ethanol production efficiencies of DBKKUY-103 during high-temperature ethanol production associated with the genes involved in DNA repair and ATP production.