Dashtiyan Amin Allah, Sepehrimanesh Masood, Tanideh Nader, Afzalpour Mohammad Esmaeil
Department of Physical Education & Sport Sciences, University of Birjand, Avini Blvd, Birjand, South Khorasan Province, Iran.
Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Khalili St, Shiraz, Fars Province, Iran.
Biochim Open. 2017 Apr 1;4:112-118. doi: 10.1016/j.biopen.2017.03.005. eCollection 2017 Jun.
The aim of this study was to investigate the effect of endurance training with and without vitamin E on the expression of p53 and Phosphatase and tension homolog (PTEN) tumor suppressor genes of prostate glands in male rats. For this purpose, 50 Sprague-Dawley male rats were randomly assigned into 5 groups: (1) control group (CON, n = 10), (2) sham (S, n = 10), (3) endurance training (ET, n = 10), (4) endurance training + vitamin E (ET + VE, n = 10), (5) vitamin E (VE, n = 10). Endurance training protocol was implemented for 6 weeks, 6 days per week, in accordance with the overload principle. To measure expression changes of p53 and PTEN genes in rats' prostate, real-time PCR method was used and HPLC method was used to measure vitamin E in this tissue. After 6 weeks of taking vitamin E, its level in all groups, except for group VE (p < 0.000) did not significantly increase. After implementing training protocol, p53 expression reduced significantly in ET group (p < 0.026). Vitamin E supplementation along with endurance training did not cause any significant change either p53 or PTEN (respectively; p < 0.2, p < 0.11). Instead, vitamin E supplementation without endurance training caused significant increase in PTEN, but did not cause any significant changes in p53 (respectively; p < 0.016, p < 0.15). These results indicate that endurance training reduces p53 and PTEN tumor suppressing genes expression, and taking vitamin E supplement could increase expression of these genes in some extent.
本研究旨在探讨有或无维生素E的耐力训练对雄性大鼠前列腺中p53和磷酸酶及张力蛋白同源物（PTEN）肿瘤抑制基因表达的影响。为此，将50只Sprague-Dawley雄性大鼠随机分为5组：（1）对照组（CON，n = 10），（2）假手术组（S，n = 10），（3）耐力训练组（ET，n = 10），（4）耐力训练+维生素E组（ET + VE，n = 10），（5）维生素E组（VE，n = 10）。根据超负荷原则，耐力训练方案实施6周，每周6天。采用实时PCR法检测大鼠前列腺中p53和PTEN基因的表达变化，采用高效液相色谱法检测该组织中的维生素E。服用维生素E 6周后，除VE组外（p < 0.000），其他各组维生素E水平均未显著升高。实施训练方案后，ET组p53表达显著降低（p < 0.026）。耐力训练同时补充维生素E对p53或PTEN均未引起任何显著变化（分别为p < 0.2，p < 0.11）。相反，不进行耐力训练而补充维生素E可使PTEN显著增加，但对p53未引起任何显著变化（分别为p < 0.016，p < 0.15）。这些结果表明，耐力训练可降低p53和PTEN肿瘤抑制基因的表达，补充维生素E在一定程度上可增加这些基因的表达。
