Department of Sport Sciences, Faculty of Educational Sciences and Psychology, University of Sistan and Baluchestan, Zahedan, Iran.
Department of Physiology and Pharmacology, Afzalipour School of Medicine, and Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman 78516-44438, Iran.
Mediators Inflamm. 2022 Aug 31;2022:1610293. doi: 10.1155/2022/1610293. eCollection 2022.
To study the effects of a six-week endurance training protocol and calcitonin gene-related peptide (CGRP) inhibition on the nuclear factor erythroid 2-related factor 2 (Nrf2) and protein kinase B (PKB) or AKT expression in the hippocampal tissue of male Wistar rats. . Building on a controlled experimental design with a posttest, 28 healthy Wistar male rats were randomly assigned to four groups ( = 7 per group), including , , , and groups. The training groups were trained for six weeks. Rats in the CGRP inhibition group received CGRP receptor antagonist daily (0.25 mg/kg) via intravenous (IV) injection. The Nrf2 and AKT (PKB) expression was measured using the real-time PCR technique.
In the endurance training group, Nrf2 expression in the hippocampal tissue was increased significantly more than in other groups ( < 0.05). There was also a significant increase in the AKT expression in the endurance training group compared to the control group ( = 0.048) and in the endurance training+CGRP inhibition compared to the control group ( = 0.012). In addition, there was no significant relationship between AKT (PKB) and Nrf2 ( = -0.27, = 28, = 0.16).
Endurance training alone has been able to increase Nrf2 and AKT (PKB) mRNA levels in the hippocampal tissue, considering that endurance training had no significant effect on AKT and Nrf2 expression after adding to CGRP inhibition.
研究为期六周的耐力训练方案和降钙素基因相关肽（CGRP）抑制对雄性 Wistar 大鼠海马组织中核因子红细胞 2 相关因子 2（Nrf2）和蛋白激酶 B（PKB）或 AKT 表达的影响。在一个具有后测的对照实验设计基础上，将 28 只健康的雄性 Wistar 大鼠随机分为四组（每组 7 只），包括 、 、 和 组。训练组进行了六周的训练。CGRP 抑制组的大鼠每天通过静脉（IV）注射接受 CGRP 受体拮抗剂（0.25mg/kg）。使用实时 PCR 技术测量 Nrf2 和 AKT（PKB）的表达。
在耐力训练组中，海马组织中的 Nrf2 表达明显高于其他组（ < 0.05）。与对照组相比，耐力训练组的 AKT 表达也显著增加（ = 0.048），与对照组相比，耐力训练+CGRP 抑制组的 AKT 表达也显著增加（ = 0.012）。此外，AKT（PKB）和 Nrf2 之间没有显著关系（ = -0.27， = 28， = 0.16）。
单独的耐力训练已经能够增加海马组织中 Nrf2 和 AKT（PKB）的 mRNA 水平，考虑到在添加 CGRP 抑制后，耐力训练对 AKT 和 Nrf2 表达没有显著影响。
