Health Radiation Research Dept, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
BMC Oral Health. 2023 Sep 27;23(1):697. doi: 10.1186/s12903-023-03408-x.
Radiation therapy is the primary treatment for neck and head cancer patients; however, it causes the development of oral mucositis accompanied by tissue structure destruction and functional alteration. This study was conducted to evaluate the effect of different doses of vitamin E as a treatment for radiationinduced oral mucositis in rat model.
35 male albino rats were randomly divided into five groups: control, untreated radiation mucositis (single dose of 20 Gy), treated radiation mucositis; radiation (single dose of 20 Gy) then vitamin E at doses of 300, 360 and 500 mg/Kg for seven days started 24 h after irradiation. Body weight and food intake were evaluated for each rat. The mucositis score was assessed every day. Rats were sacrificed once at the end of the experiment, and tongue specimens were stained with hematoxylin and eosin, anti P53 and anti Ki67 antibodies.
Results indicated more food intake and less weight reduction in vitamin E treated groups and the contrary for gamma-irradiated group. Additionally, vitamin E delayed the onset and decreased the severity and duration of mucositis. It also restored the histological structure of lingual tongue papillae. Vitamin E treated groups showed a significant higher Ki67 and lower P53 expression as compared to untreated radiation group. The overall improvement increased as vitamin E dose increased. Finally, the amelioration can be attributed to the decreased apoptosis and increased proliferation of cells.
Vitamin E especially at dose of 500 mg/Kg could be an effective treatment for radiation-induced oral mucositis.
放射疗法是头颈癌患者的主要治疗方法；然而，它会导致口腔黏膜炎的发展，伴有组织结构破坏和功能改变。本研究旨在评估不同剂量维生素 E 作为治疗大鼠模型放射性口腔黏膜炎的效果。
35 只雄性白化大鼠随机分为五组：对照组、未治疗的放射黏膜炎组（单次 20 Gy 剂量）、治疗性放射黏膜炎组；放射组（单次 20 Gy 剂量），然后在照射后 24 小时开始每天给予 300、360 和 500 mg/Kg 的维生素 E 治疗 7 天。评估每只大鼠的体重和食物摄入量。每天评估黏膜炎评分。实验结束时，一次性处死大鼠，用苏木精和伊红、抗 P53 和抗 Ki67 抗体对舌标本进行染色。
结果表明，维生素 E 治疗组的食物摄入量增加，体重减轻减少，而γ辐射组则相反。此外，维生素 E 延迟了黏膜炎的发作，降低了其严重程度和持续时间。它还恢复了舌乳头的组织学结构。与未治疗的放射组相比，维生素 E 治疗组的 Ki67 表达显著升高，而 P53 表达显著降低。随着维生素 E 剂量的增加，整体改善效果增加。最后，这种改善可以归因于细胞凋亡减少和增殖增加。
维生素 E，特别是 500 mg/Kg 的剂量，可能是治疗放射性口腔黏膜炎的有效方法。
