Ayuob Nasra, Al-Shathly Mona Ramadan, Bakhshwin Abdulaziz, Al-Abbas Nouf Saeed, Shaer Nehad A, Al Jaouni Soad, Hamed Walaa H E
Medical Histology and Cell Biology Department, Faculty of Medicine, Damietta University, Damietta, Egypt.
Biology Department, Faculty of Science, Jeddah University, Jeddah, Saudi Arabia.
Front Pharmacol. 2021 May 13;12:664248. doi: 10.3389/fphar.2021.664248. eCollection 2021.
The antioxidant, hypoglycemic, and insulin-enhancing effects of ginger and cinnamon were previously confirmed in experimental and human studies, while the combined effect of ginger and cinnamon was not thoroughly investigated until now. This study was designed to assess the antidiabetic effect of combined administration of ginger ( Roscoe) and cinnamon ( L.) in streptozotocin (STZ)-induced diabetic rats compared to metformin and to explain the mechanism behind this effect. STZ was utilized to induce diabetes mellitus in male Sprague-Dawley rats. Assessments of fasting blood glucose level (BGL), the total antioxidant capacity (TAC), serum insulin, HOMA-IR, and HOMA-β cells were performed. Pancreatic gene expression of β-catenin and p53 was assessed using RT-PCR. Assessment of histopathological alterations of pancreatic islet cells was performed using routine and immunohistochemical techniques. BGL significantly decreased ( = 0.01), while serum insulin and TAC significantly increased ( < 0.001) in both metformin- and ginger plus cinnamon-treated groups compared to the untreated diabetic group. HOMA-β cell index significantly increased ( = 0.001) in ginger plus cinnamon, indicating their enhancing effect on insulin secretion in diabetic conditions. p53 gene expression was significantly upregulated ( < 0.001), while β-catenin was insignificantly downregulated ( = 0.32) in ginger plus cinnamon-treated groups. Insulin immunoexpression in β cells significantly increased ( = 0.001, = 0.004) in metformin- and ginger plus cinnamon-treated groups, respectively. The combined administration of ginger and cinnamon has a significant hypoglycemic and antioxidant effect in STZ-induced diabetes mostly through enhancing repair of islet cells mediated upregulation of pancreatic p53 expression. Therefore, testing this effect in diabetic patients is recommended.
生姜和肉桂的抗氧化、降血糖及增强胰岛素作用先前已在实验和人体研究中得到证实，而生姜和肉桂的联合作用至今尚未得到充分研究。本研究旨在评估生姜（Roscoe）和肉桂（L.）联合给药对链脲佐菌素（STZ）诱导的糖尿病大鼠的抗糖尿病作用，并与二甲双胍进行比较，同时解释这种作用背后的机制。利用STZ诱导雄性Sprague-Dawley大鼠患糖尿病。对空腹血糖水平（BGL）、总抗氧化能力（TAC）、血清胰岛素、HOMA-IR和HOMA-β细胞进行了评估。使用RT-PCR评估胰腺β-连环蛋白和p53的基因表达。使用常规和免疫组织化学技术对胰岛细胞的组织病理学改变进行评估。与未治疗的糖尿病组相比，二甲双胍组以及生姜加肉桂治疗组的BGL显著降低（P = 0.01），而血清胰岛素和TAC显著升高（P < 0.001）。生姜加肉桂组的HOMA-β细胞指数显著升高（P = 0.001），表明它们在糖尿病状态下对胰岛素分泌有增强作用。在生姜加肉桂治疗组中，p53基因表达显著上调（P < 0.001），而β-连环蛋白下调不显著（P = 0.32）。二甲双胍组和生姜加肉桂治疗组β细胞中的胰岛素免疫表达分别显著增加（P = 0.001，P = 0.004）。生姜和肉桂联合给药对STZ诱导的糖尿病具有显著的降血糖和抗氧化作用，主要是通过增强胰岛细胞修复介导的胰腺p53表达上调。因此，建议在糖尿病患者中测试这种作用。
