a College of Life Science and Technology , Beijing University of Chemical Technology , Beijing , China.
Nat Prod Res. 2019 Aug;33(16):2359-2362. doi: 10.1080/14786419.2018.1440220. Epub 2018 Feb 16.
Chemical investigation of the rhizomes of Red. led to the identification of 27 constituents. Among them, a total of 16 compounds were obtained from for the first time, in which, and were also firstly isolated as natural products. Anti-inflammatory activity studies on 13 isolated compounds showed that β-carboline constituents, especially compounds and , significantly inhibited the expression of NO, TNF-α, IL-6 and IL-1β in lipopolysaccharide (LPS)-treated RAW264.7 macrophage cells. Moreover, western blotting analysis demonstrated that compound significantly inhibited the expression of COX-2, iNOS and the activation of NF-κB, suggesting that β-carboline structures may play an important role in inhibition of NF-κB signaling pathway, which thereby inhibits the production of inflammatory factors. The present research may not only help further elucidation of the anti-inflammatory mechanism of Red., but also provide the potential bioactive molecules for inflammatory diseases research.
对红根的根茎进行了化学研究，鉴定出 27 种成分。其中，共有 16 种化合物首次从 中获得，其中 和 也首次被分离为天然产物。对 13 种分离得到的化合物进行抗炎活性研究表明，β-咔啉类成分，尤其是化合物 和 ，可显著抑制脂多糖（LPS）处理的 RAW264.7 巨噬细胞中 NO、TNF-α、IL-6 和 IL-1β 的表达。此外，Western blot 分析表明，化合物 可显著抑制 COX-2、iNOS 的表达和 NF-κB 的激活，提示 β-咔啉结构可能在抑制 NF-κB 信号通路中发挥重要作用，从而抑制炎症因子的产生。本研究不仅有助于进一步阐明红根的抗炎机制，而且为炎症性疾病的研究提供了潜在的生物活性分子。
