Fujian Vocational College of Bioengineering, Fuzhou 350007, China.
Institute of Food Science & Technology, Fuzhou University, Fuzhou 350108, China.
Molecules. 2022 Jul 22;27(15):4685. doi: 10.3390/molecules27154685.
The rhizomes of are commonly consumed as food and also used as medicine. However, the metabolic profile of has not been fully revealed yet. Recently, we developed a novel evergreen species of P. sibiricum. The objectives of this study were to compare the metabolic profiles of two types of , i.e., the newly developed evergreen type (Gtype) and a wide-type (Wtype), by using UHPLC-Q-Orbitrap-MS-based untargeted metabolomics approach. A total of 263 and 258 compounds in the positive and negative modes of the mass spectra were tentatively identified. Distinctively different metabolomic profiles of these two types of were also revealed by principal component analysis (PCA) and principal coordinates analysis (PCoA). Furthermore, by using partial least squares discriminant analysis (PLS-DA) modeling, it was found that, as compared with Wtype, Gtype samples had significantly higher content of oxyberberine, proliferin, alpinetin, and grandisin. On the other hand, 15 compounds, including herniarin, kaempferol 7-neohesperidoside, benzyl beta-primeveroside, vanillic acid, biochanin A, neoschaftoside, benzyl gentiobioside, cornuside, hydroxytyrosol-glucuronide, apigenin-pentosyl-glucoside, obacunone, 13-alpha-(21)-epoxyeurycomanone, vulgarin, digitonin, and 3-formylindole, were discovered to have higher abundance in Wtype samples. These distinguishing metabolites suggest the different beneficial health potentials and flavor attributes of the two types of rhizomes.
是一种根茎常被作为食物食用，也被用作药材。然而，其代谢物谱尚未完全揭示。最近，我们开发了一种新的西伯利亚远志常绿品种。本研究的目的是通过超高效液相色谱-四极杆轨道阱质谱（UHPLC-Q-Orbitrap-MS）非靶向代谢组学方法比较两种类型根茎的代谢物谱，即新开发的常绿型（G 型）和宽叶型（W 型）。在正、负离子模式下，共鉴定出 263 种和 258 种化合物。通过主成分分析（PCA）和主坐标分析（PCoA）也揭示了这两种类型根茎的代谢物图谱明显不同。此外，通过偏最小二乘判别分析（PLS-DA）模型发现，与 W 型相比，G 型样品中氧小檗碱、增殖素、白杨素和大根香叶素的含量明显较高。另一方面，与 W 型相比，15 种化合物，包括茵陈素、山奈酚 7-新橙皮糖苷、苯甲基β-首樱草糖苷、香草酸、大豆苷元、新甘草苷、苯甲基龙胆二糖苷、山茱萸苷、羟基酪醇葡萄糖醛酸苷、芹菜素-五糖葡萄糖苷、obacunone、13-α-（21）-表氧化鹅掌楸酮、芫花素、digitonin 和 3-甲酰基吲哚，在 W 型样品中的丰度更高。这些具有区别性的代谢物表明了两种类型根茎具有不同的健康潜力和风味特征。
