Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, South Korea.
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki, Japan.
Mol Ecol Resour. 2018 May;18(3):646-655. doi: 10.1111/1755-0998.12768. Epub 2018 Mar 3.
Monogononta is the most speciose class of rotifers, with more than 2,000 species. The monogonont genus Brachionus is widely distributed at a global scale, and a few of its species are commonly used as ecological and evolutionary models to address questions related to aquatic ecology, cryptic speciation, evolutionary ecology, the evolution of sex and ecotoxicology. With the importance of Brachionus species in many areas of research, it is remarkable that the genome has not been characterized. This study aims to address this lacuna by presenting, for the first time, the whole-genome assembly of the freshwater species Brachionus calyciflorus. The total length of the assembled genome was 129.6 Mb, with 1,041 scaffolds. The N50 value was 786.6 kb, and the GC content was 24%. A total of 16,114 genes were annotated with repeat sequences, accounting for 21% of the assembled genome. This assembled genome may form a basis for future studies addressing key questions on the evolution of monogonont rotifers. It will also provide the necessary molecular resources to mechanistically investigate ecophysiological and ecotoxicological responses.
Monogononta 是轮虫中最具物种多样性的类群，拥有超过 2000 个物种。Monogononta 属的 Brachionus 广泛分布于全球范围内，其少数物种被广泛用作生态和进化模型，以解决与水生生态学、隐种形成、进化生态学、性的进化和生态毒理学相关的问题。鉴于 Brachionus 物种在许多研究领域的重要性，令人惊讶的是，其基因组尚未得到表征。本研究旨在通过首次呈现淡水物种 Brachionus calyciflorus 的全基因组组装来填补这一空白。组装基因组的总长度为 129.6 Mb，有 1041 个支架。N50 值为 786.6 kb，GC 含量为 24%。总共注释了 16114 个带有重复序列的基因，占组装基因组的 21%。这个组装的基因组可能为未来解决单巢轮虫进化的关键问题的研究奠定基础。它还将为机械地研究生理生态和生态毒理学反应提供必要的分子资源。
