Kim Duck-Hyun, Byeon Eunjin, Kim Min-Sub, Lee Young Hwan, Park Jun Chul, Hagiwara Atsushi, Lee Jae-Seong
Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon, 16419, South Korea.
Départment Des Sciences, Université Sainte-Anne, Church Point, NS, B0W 1M0, Canada.
Mar Biotechnol (NY). 2022 Mar;24(1):226-242. doi: 10.1007/s10126-022-10102-6. Epub 2022 Mar 9.
The marine rotifer Brachionus manjavacas is widely used in ecological, ecotoxicological, and ecophysiological studies. The reference genome of B. manjavacas is a good starting point to uncover the potential molecular mechanisms of responses to various environmental stressors. In this study, we assembled the whole-genome sequence (114.1 Mb total, N50 = 6.36 Mb) of B. manjavacas, consisting of 61 contigs with 18,527 annotated genes. To elucidate the potential ligand-receptor signaling pathways in marine Brachionus rotifers in response to environmental signals, we identified 310 G protein-coupled receptor (GPCR) genes in the B. manjavacas genome after comparing them with three other species, including the minute rotifer Proales similis, Drosophila melanogaster, and humans (Homo sapiens). The 310 full-length GPCR genes were categorized into five distinct classes: A (262), B (26), C (7), F (2), and other (13). Most GPCR gene families showed sporadic evolutionary processes, but some classes were highly conserved between species as shown in the minute rotifer P. similis. Overall, these results provide potential clues for in silico analysis of GPCR-based signaling pathways in the marine rotifer B. manjavacas and will expand our knowledge of ligand-receptor signaling pathways in response to various environmental signals in rotifers.
海洋轮虫曼氏臂尾轮虫（Brachionus manjavacas）广泛应用于生态学、生态毒理学和生态生理学研究。曼氏臂尾轮虫的参考基因组是揭示其对各种环境应激源潜在分子反应机制的良好起点。在本研究中，我们组装了曼氏臂尾轮虫的全基因组序列（总计114.1 Mb，N50 = 6.36 Mb），该序列由61个重叠群组成，含有18,527个注释基因。为了阐明海洋臂尾轮虫对环境信号潜在的配体 - 受体信号通路，在将曼氏臂尾轮虫基因组与其他三个物种（包括微小轮虫相似原臂尾轮虫（Proales similis）、黑腹果蝇（Drosophila melanogaster）和人类（Homo sapiens））进行比较后，我们在曼氏臂尾轮虫基因组中鉴定出310个G蛋白偶联受体（GPCR）基因。这310个全长GPCR基因被分为五个不同类别：A类（262个）、B类（26个）、C类（7个）、F类（2个）和其他类（13个）。大多数GPCR基因家族显示出零散的进化过程，但如微小轮虫相似原臂尾轮虫所示，有些类别在物种间高度保守。总体而言，这些结果为基于计算机模拟分析曼氏臂尾轮虫中基于GPCR的信号通路提供了潜在线索，并将扩展我们对轮虫中响应各种环境信号的配体 - 受体信号通路的认识。
