Akroyd J E, Clayson E, Higgins N P
Nucleic Acids Res. 1986 Sep 11;14(17):6901-14. doi: 10.1093/nar/14.17.6901.
The gam gene of bacteriophage Mu encodes a protein which protects linear double stranded DNA from exonuclease degradation in vitro and in vivo. We purified the Mu gam gene product to apparent homogeneity from cells in which it is over-produced from a plasmid clone. The purified protein is a dimer of identical subunits of 18.9 kd. It can aggregate DNA into large, rapidly sedimenting complexes and is a potent exonuclease inhibitor when bound to DNA. The N-terminal amino acid sequence of the purified protein was determined by automated degradation and the nucleotide sequence of the Mu gam gene is presented to accurately map its position in the Mu genome.
噬菌体Mu的gam基因编码一种蛋白质，该蛋白质在体外和体内可保护线性双链DNA免受核酸外切酶的降解。我们从通过质粒克隆过量表达gam基因的细胞中纯化出了达到表观均一性的Mu gam基因产物。纯化后的蛋白质是由两个18.9kd相同亚基组成的二聚体。它能使DNA聚集成大的、快速沉降的复合物，并且在与DNA结合时是一种有效的核酸外切酶抑制剂。通过自动降解确定了纯化蛋白质的N端氨基酸序列，并给出了Mu gam基因的核苷酸序列，以精确确定其在Mu基因组中的位置。
