Département de Biologie et Pathologie Médicales, Service de Pathologie Morphologique, Villejuif, France.
Service de Biostatistique et d'Epidémiologie, Gustave Roussy Cancer Campus, Villejuif, France.
Am J Clin Pathol. 2018 Mar 7;149(4):344-351. doi: 10.1093/ajcp/aqx161.
The current challenge for the various digital whole-slide imaging (WSI) systems is to be definitively validated for diagnostic purposes. We designed a concordance study between glass slide and digital slide diagnosis in real-life conditions, coupled with an ergonomic study.
Three senior pathologists evaluated, first in glass slides and then in digital slides, 119 biopsy cases, including 749 slides, with 332 H&E saffron stains and 417 additional techniques, mainly immunohistochemistry.
All digital slides, including specially stained slides, were interpretable. Concordance between glass slides and digital slides was observed in 87.4% of cases. Minor discordances were observed in 12 (10.1%) cases and major discordances, with therapeutic impact, in three (2.5%), including one related to WSI. The satisfaction of participants was high and increased with time.
Our study confirms the feasibility and accuracy of WSI diagnosis, even for cases having multiple samples and requiring special staining techniques, such as immunohistochemistry and in situ hybridization.
目前各种数字全玻片成像（WSI）系统都面临着一个挑战，即必须针对诊断目的进行明确验证。我们设计了一项在实际条件下进行的玻璃片与数字片诊断之间的一致性研究，并结合了一项人体工程学研究。
3 名资深病理学家首先在玻璃片上，然后在数字片上评估了 119 例活检病例，包括 749 张玻片，其中 332 张为 H&E 藏红花染色，417 张为其他技术，主要是免疫组织化学染色。
所有数字玻片，包括特殊染色玻片，均可进行解读。玻璃片和数字片之间的一致性在 87.4%的病例中得到了观察。在 12 例（10.1%）病例中观察到了轻微的不一致，在 3 例（2.5%）病例中观察到了主要的不一致，其中一例与 WSI 相关。参与者的满意度很高，且随着时间的推移而增加。
我们的研究证实了 WSI 诊断的可行性和准确性，即使是对于需要多个样本和特殊染色技术（如免疫组织化学和原位杂交）的病例也是如此。
