Brandão Ildnay de Souza Lima, Oliveira-Moraes Heloiza Maria da Silva, Souza Motta Cristina Maria de, Oliveira Neiva Tinti de, Magalhães Oliane Maria Correia
Universidade Federal de Pernambuco, Centro de Ciências Biológicas, Departamento de Micologia, Cidade Universitária, PE, Brazil.
Universidade Federal de Pernambuco, Centro de Ciências Biológicas Rua Departamento de Micologia, Cidade Universitária, PE, Brazil.
Braz J Microbiol. 2018 Jul-Sep;49(3):675-682. doi: 10.1016/j.bjm.2017.10.004. Epub 2018 Feb 13.
Aspergillus fumigatus is an opportunistic saprobe fungus that accounts for 90% of cases of pulmonary aspergillosis in immunosuppressed patients and is known for its angiotropism. When it reaches the respiratory tract, A. fumigatus interacts with structural components and blood vessels of the lungs, such as elastin. To understand the effect of this structural component, we examined the effect of elastin on the production and development of the biofilm of A. fumigatus. In RPMI containing 10mg/mL of elastin, a significant increase (absorbance p<0.0001; dry weight p<0.0001) in the production of biofilm was observed in comparison to when RPMI was used alone, reaching a maximum growth of 18.8mg (dry weight) of biofilm in 72h. In addition, elastin stimulates the production (p=0.0042) of extracellular matrix (ECM) and decreases (p=0.005) the hydrophobicity during the development of the biofilm. These results suggest that elastin plays an important role in the growth of A. fumigatus and that it participates in the formation of thick biofilm.
烟曲霉是一种机会性腐生真菌，在免疫抑制患者的肺曲霉病病例中占90%，并以其血管嗜性而闻名。当它到达呼吸道时，烟曲霉会与肺部的结构成分和血管相互作用，如弹性蛋白。为了了解这种结构成分的作用，我们研究了弹性蛋白对烟曲霉生物膜产生和发育的影响。在含有10mg/mL弹性蛋白的RPMI培养基中，与单独使用RPMI相比，观察到生物膜产生显著增加（吸光度p<0.0001；干重p<0.0001），在72小时内生物膜的最大生长量达到18.8mg（干重）。此外，弹性蛋白在生物膜发育过程中刺激细胞外基质（ECM）的产生（p=0.0042）并降低（p=0.005）疏水性。这些结果表明弹性蛋白在烟曲霉的生长中起重要作用，并且它参与厚生物膜的形成。
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