Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
J Mycol Med. 2012 Jun;22(2):167-72. doi: 10.1016/j.mycmed.2012.03.002. Epub 2012 May 23.
To evaluate the relationship between elastase activity of different Aspergillus fumigatus (A. fumigatus) isolates and their pathogenicity in mice.
The animals were intravenously (IV) infected with 1×10(6) conidia/mouse. The elastase activity in mice sera and survival time were determined. All animals were sacrified at the end of the fourth week, and autopsies were performed for histopathological examination. The tissue sections were stained with Periodic Acid Schiff (PAS) and Verhoeff's techniques.
The highest elastase activity was associated with the sera of infected mice with elastase-producing A. fumigatus AIR78 as standard strain (0.18±0.02mm), followed by infected animals with elastase-producing isolates (0.16±0.02mm) and control mice (0.14±0.02mm). The elastase activity index (EAI) was determined in value of 0.92±0.27. The survival times in mice infected with elastase-producing isolate and in control group were 21.6 and 30 days, respectively. Histopathological studies showed that the heart and kidneys were more infected by Aspergillus hyphae than other tissues.
It was concluded that there is a significant relationship between elastase activity and survival time in mice with aspergillosis.
评估不同烟曲霉（A. fumigatus）分离株弹性蛋白酶活性与其在小鼠中的致病性之间的关系。
动物经静脉（IV）感染 1×10(6)个孢子/只。测定小鼠血清中的弹性蛋白酶活性和存活时间。所有动物在第四周结束时处死，并进行尸检进行组织病理学检查。组织切片用过碘酸希夫（PAS）和Verhoeff 技术染色。
以产弹性蛋白酶的 A. fumigatus AIR78 为标准菌株的血清中弹性蛋白酶活性最高（0.18±0.02mm），其次是产弹性蛋白酶的分离株感染动物（0.16±0.02mm）和对照小鼠（0.14±0.02mm）。弹性蛋白酶活性指数（EAI）为 0.92±0.27。产弹性蛋白酶分离株感染小鼠和对照组的存活时间分别为 21.6 天和 30 天。组织病理学研究表明，心脏和肾脏比其他组织更容易被曲霉菌丝感染。
曲霉病小鼠的弹性蛋白酶活性与存活时间之间存在显著关系。
