用于活细胞中 SO 衍生物快速检测的线粒体靶向比色和比率荧光探针的设计。
Design of mitochondria-targeted colorimetric and ratiometric fluorescent probes for rapid detection of SO derivatives in living cells.
机构信息
Key Laboratory of Medicinal Chemistry, and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
Institute of Molecular Science, Shanxi University, Taiyuan 030006, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 May 5;196:215-221. doi: 10.1016/j.saa.2018.01.066. Epub 2018 Feb 20.
Two mitochondria-targeted colorimetric and ratiometric fluorescent probes for SO derivatives were constructed based on the SO derivatives-triggered Michael addition reaction. The probes exhibit high specificity toward HSO/SO by interrupting their conjugation system resulting in a large ratiometric blue shift of 46-121nm in their emission spectrum. The two well-resolved emission bands can ensure accurate detection of HSO. The detection limits were calculated to be 1.09 and 1.35μM. Importantly, probe 1 and probe 2 were successfully used to fluorescence ratiometric imaging of endogenous HSO in BT-474 cells.
基于 SO 衍生物引发的迈克尔加成反应，构建了两种靶向线粒体的比色和比率荧光探针用于检测 SO 衍生物。这些探针通过中断它们的共轭体系对 HSO/SO 具有高特异性，导致其发射光谱中出现 46-121nm 的大比率蓝移。两个良好分辨的发射带可以确保对 HSO 的准确检测。检测限计算值分别为 1.09 和 1.35μM。重要的是，探针 1 和探针 2 成功地用于 BT-474 细胞内源性 HSO 的荧光比率成像。
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