一种用于检测活细胞内源性SO衍生物的线粒体靶向荧光探针。
A mitochondria-targeted fluorescent probe for the detection of endogenous SO derivatives in living cells.
作者信息
Yan Ye-Hao, Wu Qiu-Rong, Che Qiao-Ling, Ding Man-Man, Xu Min, Miao Jun-Ying, Zhao Bao-Xiang, Lin Zhao-Min
机构信息
Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China.
School of Life Science, Shandong University, Qingdao 266237, P.R. China.
出版信息
Analyst. 2020 Apr 14;145(8):2937-2944. doi: 10.1039/d0an00086h.
A unique fluorescent probe (ZACA) for the monitoring of SO2 derivatives was developed from coumarin and benzoindoles based on FRET and ICT. ZACA exhibited an active emission signal, large Stokes shift, wide emission window distance, and high photostability. It also possessed many advantages in the ratiometric detection of HSO3-/SO32- including low detection limit and high selectivity and sensitivity. Importantly, ZACA was successfully applied in the ratiometric detection of endogenous HSO3-/SO32- in living cells with excellent cellular imaging capability (1 μM) and mitochondria-targeting ability (co-localization coefficient: 0.91).
基于荧光共振能量转移（FRET）和分子内电荷转移（ICT）原理，从香豆素和苯并吲哚出发，开发了一种用于监测二氧化硫衍生物的独特荧光探针（ZACA）。ZACA表现出活性发射信号、大斯托克斯位移、宽发射窗口距离和高光稳定性。它在亚硫酸氢根/亚硫酸根离子的比率检测中也具有许多优点，包括低检测限、高选择性和高灵敏度。重要的是，ZACA已成功应用于活细胞内源性亚硫酸氢根/亚硫酸根离子的比率检测，具有出色的细胞成像能力（1 μM）和线粒体靶向能力（共定位系数：0.91）。
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